| http://purl.uniprot.org/citations/25959678 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25959678 | http://www.w3.org/2000/01/rdf-schema#comment | "The ATP-dependent proton pump V-ATPase ensures low intralysosomal pH, which is essential for lysosomal hydrolase activity. Based on studies with the V-ATPase inhibitor BafilomycinA1, lysosomal acidification is also thought to be required for fusion with incoming vesicles from the autophagic and endocytic pathways. Here we show that loss of V-ATPase subunits in the Drosophila fat body causes an accumulation of non-functional lysosomes, leading to a block in autophagic flux. However, V-ATPase-deficient lysosomes remain competent to fuse with autophagosomes and endosomes, resulting in a time-dependent formation of giant autolysosomes. In contrast, BafilomycinA1 prevents autophagosome-lysosome fusion in these cells, and this defect is phenocopied by depletion of the Ca(2+) pump SERCA, a secondary target of this drug. Moreover, activation of SERCA promotes fusion in a BafilomycinA1-sensitive manner. Collectively, our results indicate that lysosomal acidification is not a prerequisite for fusion, and that BafilomycinA1 inhibits fusion independent of its effect on lysosomal pH."xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms8007"xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/author | "Juhasz G."xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/author | "Neufeld T.P."xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/author | "Nagy P."xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/author | "Mauvezin C."xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/pages | "7007"xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/title | "Autophagosome-lysosome fusion is independent of V-ATPase-mediated acidification."xsd:string |
| http://purl.uniprot.org/citations/25959678 | http://purl.uniprot.org/core/volume | "6"xsd:string |
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