| http://purl.uniprot.org/citations/25979886 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25979886 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25979886 | http://www.w3.org/2000/01/rdf-schema#comment | "Two glucoamylase-like genes, TVN1315 and Ta0286, from the archaea Thermoplasma volcanium and T. acidophilum, respectively, were expressed in Escherichia coli. The gene products, TVN1315 and Ta0286, were identified as archaeal trehalases. These trehalases belong to the CAZy database family GH15, although they have putative (α/α)6 barrel catalytic domain structures similar to those of GH37 and GH65 family trehalases from other organisms. These newly identified trehalases function within a narrow range of acidic pH values (pH 3.2 to 4.0) and at high temperatures (50 to 60°C), and these enzymes display Km values for trehalose higher than those observed for typical trehalases. These enzymes were inhibited by validamycin A; however, the inhibition constants (Ki) were higher than those of other trehalases. Three TVN1315 mutants, corresponding to E408Q, E571Q, and E408Q/E571Q mutations, showed reduced activity, suggesting that these two glutamic acid residues are involved in trehalase catalysis in a manner similar to that of glucoamylase. To date, TVN1315 and Ta0286 are the first archaeal trehalases to be identified, and this is the first report of the heterologous expression of GH15 family trehalases. The identification of these trehalases could extend our understanding of the relationships between the structure and function of GH15 family enzymes as well as glycoside hydrolase family enzymes; additionally, these enzymes provide insight into archaeal trehalose metabolism."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.00956-15"xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.00956-15"xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Amemiya M."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Amemiya M."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Honda S."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Honda S."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Ishida S.N."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Ishida S.N."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Kawakita M."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Kawakita M."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Oyama F."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Oyama F."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Sakaguchi M."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Sakaguchi M."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Shimodaira S."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Shimodaira S."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Sugahara Y."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/author | "Sugahara Y."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/25979886 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |