| http://purl.uniprot.org/citations/25990725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25990725 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/25990725 | http://www.w3.org/2000/01/rdf-schema#comment | "Efficient pre-replication complex (pre-RC) formation on chromatin templates is crucial for the maintenance of genome integrity. However, the regulation of chromatin dynamics during this process has remained elusive. We found that a conserved protein, GRWD1 (glutamate-rich WD40 repeat containing 1), binds to two representative replication origins specifically during G1 phase in a CDC6- and Cdt1-dependent manner, and that depletion of GRWD1 reduces loading of MCM but not CDC6 and Cdt1. Furthermore, chromatin immunoprecipitation coupled with high-throughput sequencing (Seq) revealed significant genome-wide co-localization of GRWD1 with CDC6. We found that GRWD1 has histone-binding activity. To investigate the effect of GRWD1 on chromatin architecture, we used formaldehyde-assisted isolation of regulatory elements (FAIRE)-seq or FAIRE-quantitative PCR analyses, and the results suggest that GRWD1 regulates chromatin openness at specific chromatin locations. Taken together, these findings suggest that GRWD1 may be a novel histone-binding protein that regulates chromatin dynamics and MCM loading at replication origins."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkv509"xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkv509"xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Fujita M."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Fujita M."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Kiyono T."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Kiyono T."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Watanabe S."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Watanabe S."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Yoshida K."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Yoshida K."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Kurumizaka H."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Kurumizaka H."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Yugawa T."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Yugawa T."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Sugimoto N."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Sugimoto N."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Aizawa M."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Aizawa M."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Osano S."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Osano S."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Maehara K."xsd:string |
| http://purl.uniprot.org/citations/25990725 | http://purl.uniprot.org/core/author | "Maehara K."xsd:string |