Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/26017478http://www.w3.org/2000/01/rdf-schema#comment"

Background

MicroRNA-19a (miR-19a), an oncogenic microRNA, has been recently reported to target CD22 in B cell lymphoma cell lines, but its role in inflammatory response is unclear. CD22 is a negative regulator for BCR signaling, and we hypothesize that miR-19a regulates B cell response by targeting CD22 in sepsis.

Material and methods

In order to determine whether miR-19a-CD22 pathway was involved in sepsis, and what role it played in the regulatory mechanisms, we detected the levels of miR-19a in B cells obtained from patients with sepsis, and measured the levels of miR-19a and CD22 expression in B cells activated by LPS in vitro. Additionally, we investigated the correlation between miR-19a and CD22, as well as the influence of this pathway on BCR signaling, in transfected B cells.

Results

We found that septic patients displayed up-regulated miR-19a in B cells. In vitro, miR-19a was increased in activated B cells, with CD22 expression initially enhanced but subsequently decreased. Moreover, overexpression of miR-19a resulted in an amplified BCR signaling, while overexpression of CD22 attenuated the effect of miR-19a and increased its expression.

Conclusions

Our study demonstrated that miR-19a and CD22 comprised a feedback loop for B cell response in sepsis, providing a potential therapeutic target to recover the immune homeostasis."xsd:string
http://purl.uniprot.org/citations/26017478http://purl.org/dc/terms/identifier"doi:10.12659/msm.894321"xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/author"Cai X."xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/author"Jiang Y."xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/author"Ma D."xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/author"Zhou H."xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/author"Chen Z.K."xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/name"Med Sci Monit"xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/pages"1548-1555"xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/title"MicroRNA-19a and CD22 Comprise a Feedback Loop for B Cell Response in Sepsis."xsd:string
http://purl.uniprot.org/citations/26017478http://purl.uniprot.org/core/volume"21"xsd:string
http://purl.uniprot.org/citations/26017478http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/26017478
http://purl.uniprot.org/citations/26017478http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/26017478
http://purl.uniprot.org/uniprot/P20273#attribution-4EE32D6D213FB2FCE54AD4C6107E5606http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/#_B7Z6Y6-mappedCitation-26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/#_Q0EAF5-mappedCitation-26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/#_O95700-mappedCitation-26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/#_P20273-mappedCitation-26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/#_O60926-mappedCitation-26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/#_Q9H5X8-mappedCitation-26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/#_Q9UQB1-mappedCitation-26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/#_Q9UQB2-mappedCitation-26017478http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26017478
http://purl.uniprot.org/uniprot/Q9UQB1http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/26017478
▶