| http://purl.uniprot.org/citations/26017780 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26017780 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26017780 | http://www.w3.org/2000/01/rdf-schema#comment | "A proteome wide analysis was performed in Escherichia coli to identify the impact on protein N-termini of actinonin, an antibiotic specifically inhibiting peptide deformylase (PDF). A strategy and tool suite (SILProNaQ) was employed to provide large-scale quantitation of N-terminal modifications. In control conditions, more than 1000 unique N-termini were identified with 56% showing initiator methionine removal. Additional modifications corresponded to partial or complete Nα-acetylation (10%) and N-formyl retention (5%). Among the proteins undergoing these N-terminal modifications, 140 unique N-termini from translocated membrane proteins were highlighted. The very early time-course impact of actinonin was followed after addition of bacteriostatic concentrations of the drug. Under these conditions, 26% of all proteins did not undergo deformylation any longer after 10 min, a value reaching more than 60% of all characterized proteins after 40 min of treatment. The N-formylation ratio measured on individual proteins increased with the same trend. Upon early PDF inhibition, two major categories of proteins retained their N-formyl group: a large number of inner membrane proteins and many proteins involved in protein synthesis including factors assisting the nascent chains in early cotranslational events. All MS data have been deposited in the ProteomeXchange with identifiers PXD001979, PXD002012 and PXD001983 (http://proteomecentral.proteomexchange.org/dataset/PXD001979, http://proteomecentral.proteomexchange.org/dataset/PXD002012 and http://proteomecentral.proteomexchange.org/dataset/PXD001983)."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.201500027"xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.201500027"xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/author | "Bienvenut W.V."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/author | "Bienvenut W.V."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/author | "Giglione C."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/author | "Giglione C."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/author | "Meinnel T."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/author | "Meinnel T."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/pages | "2503-2518"xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/pages | "2503-2518"xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/title | "Proteome-wide analysis of the amino terminal status of Escherichia coli proteins at the steady-state and upon deformylation inhibition."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/title | "Proteome-wide analysis of the amino terminal status of Escherichia coli proteins at the steady-state and upon deformylation inhibition."xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/26017780 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26017780 |
| http://purl.uniprot.org/citations/26017780 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26017780 |
| http://purl.uniprot.org/citations/26017780 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26017780 |
| http://purl.uniprot.org/citations/26017780 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26017780 |