| http://purl.uniprot.org/citations/26039452 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26039452 | http://www.w3.org/2000/01/rdf-schema#comment | "Fibroblast growth factor 15 (FGF15) has been proposed as a postprandial hormone that signals from intestine to liver to regulate bile acid and carbohydrate homeostasis. However, detecting FGF15 in blood using conventional techniques has proven difficult. Here, we describe a stable isotope standards and capture by anti-peptide antibodies (SISCAPA) assay that combines immuno-enrichment with selected reaction monitoring (SRM) mass spectrometry to overcome this issue. Using this assay, we show that FGF15 circulates in plasma in an FXR and circadian rhythm-dependent manner at concentrations that activate its receptor. Consistent with the proposed endocrine role for FGF15 in liver, mice lacking hepatocyte expression of the obligate FGF15 co-receptor, β-Klotho, have increased bile acid synthesis and reduced glycogen storage despite having supraphysiological plasma FGF15 concentrations. Collectively, these data demonstrate that FGF15 functions as a hormone and highlight the utility of SISCAPA-SRM as a sensitive assay for detecting low-abundance proteins in plasma."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cmet.2015.05.004"xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/author | "Ding X."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/author | "Mangelsdorf D.J."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/author | "Katafuchi T."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/author | "Mirzaei H."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/author | "Esterhazy D."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/author | "Kliewer S.A."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/author | "Lemoff A."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/author | "Sondhi V."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/name | "Cell Metab"xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/pages | "898-904"xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/title | "Detection of FGF15 in plasma by stable isotope standards and capture by anti-peptide antibodies and targeted mass spectrometry."xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://purl.uniprot.org/core/volume | "21"xsd:string |
| http://purl.uniprot.org/citations/26039452 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26039452 |
| http://purl.uniprot.org/citations/26039452 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26039452 |
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| http://purl.uniprot.org/uniprot/Q99N32 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/26039452 |