| http://purl.uniprot.org/citations/26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26051888 | http://www.w3.org/2000/01/rdf-schema#comment | "Replication origins are under tight regulation to ensure activation occurs only once per cell cycle [1, 2]. Origin re-firing in a single S phase leads to the generation of DNA double-strand breaks (DSBs) and activation of the DNA damage checkpoint [2-7]. If the checkpoint is blocked, cells enter mitosis with partially re-replicated DNA that generates chromosome breaks and fusions [5]. These types of chromosomal aberrations are common in numerous human cancers, suggesting that re-replication events contribute to cancer progression. It was proposed that fork instability and DSBs formed during re-replication are the result of head-to-tail collisions and collapse of adjacent replication forks [3]. However, previously studied systems lack the resolution to determine whether the observed DSBs are generated at sites of fork collisions. Here, we utilize the Drosophila ovarian follicle cells, which exhibit re-replication under precise developmental control [8-10], to model the consequences of re-replication at actively elongating forks. Re-replication occurs from specific replication origins at six genomic loci, termed Drosophila amplicons in follicle cells (DAFCs) [10-12]. Precise developmental timing of DAFC origin firing permits identification of forks at defined points after origin initiation [13, 14]. Here, we show that DAFC re-replication causes fork instability and generates DSBs at sites of potential fork collisions. Immunofluorescence and ChIP-seq demonstrate the DSB marker γH2Av is enriched at elongating forks. Fork progression is reduced in the absence of DNA damage checkpoint components and nonhomologous end-joining (NHEJ), but not homologous recombination. NHEJ appears to continually repair forks during re-replication to maintain elongation."xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cub.2015.04.058"xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://purl.uniprot.org/core/author | "Barrasa M.I."xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://purl.uniprot.org/core/author | "Orr-Weaver T.L."xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://purl.uniprot.org/core/author | "Alexander J.L."xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26051888 | http://purl.uniprot.org/core/name | "Curr Biol"xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://purl.uniprot.org/core/pages | "1654-1660"xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://purl.uniprot.org/core/title | "Replication fork progression during re-replication requires the DNA damage checkpoint and double-strand break repair."xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://purl.uniprot.org/core/volume | "25"xsd:string |
| http://purl.uniprot.org/citations/26051888 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26051888 |
| http://purl.uniprot.org/citations/26051888 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26051888 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KH25-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_A4V0X0-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_D3DMK7-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_P08985-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_O61267-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_O61661-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_Q27297-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_Q2XXV4-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_Q59DY7-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_Q8MS06-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_Q7YU37-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |
| http://purl.uniprot.org/uniprot/#_Q9GTU6-mappedCitation-26051888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26051888 |