| http://purl.uniprot.org/citations/26063423 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26063423 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26063423 | http://www.w3.org/2000/01/rdf-schema#comment | "Theiler's murine encephalomyelitis virus (TMEV) is a member of the genus Cardiovirus in the Picornaviridae, a family of positive-sense single-stranded RNA viruses. Previously, we demonstrated that in the related cardiovirus, Encephalomyocarditis virus, a programmed-1 ribosomal frameshift (1 PRF) occurs at a conserved G_GUU_UUU sequence within the 2B-encoding region of the polyprotein open reading frame (ORF). Here we show that-1 PRF occurs at a similar site during translation of the TMEV genome. In addition, we demonstrate that a predicted 3= RNA stem-loop structure at a noncanonical spacing downstream of the shift site is required for efficient frameshifting in TMEV and that frameshifting also requires virus infection. Mutating the G_GUU_UUU shift site to inhibit frameshifting results in an attenuated virus with reduced growth kinetics and a small-plaque phenotype. Frameshifting in the virus context was found to be extremely efficient at 74 to 82%, which, to our knowledge, is the highest frameshifting efficiency recorded to date for any virus. We propose that highly efficient-1 PRF in TMEV provides a mechanism to escape the confines of equimolar expression normally inherent in the single-polyprotein expression strategy of picornaviruses."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.01043-15"xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.01043-15"xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Ling R."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Ling R."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Bell S."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Bell S."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Brierley I."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Brierley I."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Olspert A."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Olspert A."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Finch L.K."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Finch L.K."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Firth A.E."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Firth A.E."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Lardinois C."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Lardinois C."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Loughran G."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Loughran G."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Michiels T."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Michiels T."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Napthine S."xsd:string |
| http://purl.uniprot.org/citations/26063423 | http://purl.uniprot.org/core/author | "Napthine S."xsd:string |