| http://purl.uniprot.org/citations/26071590 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26071590 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26071590 | http://www.w3.org/2000/01/rdf-schema#comment | "The haloacid dehalogenase (HAD)-like enzymes comprise a large superfamily of phosphohydrolases present in all organisms. The Saccharomyces cerevisiae genome encodes at least 19 soluble HADs, including 10 uncharacterized proteins. Here, we biochemically characterized 13 yeast phosphatases from the HAD superfamily, which includes both specific and promiscuous enzymes active against various phosphorylated metabolites and peptides with several HADs implicated in detoxification of phosphorylated compounds and pseudouridine. The crystal structures of four yeast HADs provided insight into their active sites, whereas the structure of the YKR070W dimer in complex with substrate revealed a composite substrate-binding site. Although the S. cerevisiae and Escherichia coli HADs share low sequence similarities, the comparison of their substrate profiles revealed seven phosphatases with common preferred substrates. The cluster of secondary substrates supporting significant activity of both S. cerevisiae and E. coli HADs includes 28 common metabolites that appear to represent the pool of potential activities for the evolution of novel HAD phosphatases. Evolution of novel substrate specificities of HAD phosphatases shows no strict correlation with sequence divergence. Thus, evolution of the HAD superfamily combines the conservation of the overall substrate pool and the substrate profiles of some enzymes with remarkable biochemical and structural flexibility of other superfamily members."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.657916"xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.657916"xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Brown G."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Brown G."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Edwards A.M."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Edwards A.M."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Evdokimova E."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Evdokimova E."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Flick R."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Flick R."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Joachimiak A."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Joachimiak A."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Koonin E.V."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Koonin E.V."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Hanson A.D."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Hanson A.D."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Makarova K.S."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Makarova K.S."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Kuznetsova E."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Kuznetsova E."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Nocek B."xsd:string |
| http://purl.uniprot.org/citations/26071590 | http://purl.uniprot.org/core/author | "Nocek B."xsd:string |