| http://purl.uniprot.org/citations/2610270 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2610270 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2610270 | http://www.w3.org/2000/01/rdf-schema#comment | "SP-A, a glycoprotein of pulmonary surfactant, consists of an NH2-terminal domain containing a collagen-like sequence and a COOH-terminal domain with sequence homology to several Ca2(+)-dependent lectins. We have compared the size, thermal stability, and secondary structure of recombinant SP-A, the product of a fibroblast line transfected with a single human gene encoding SP-A, with natural SP-A isolated from canine and human lungs. Our results suggest both recombinant and natural SP-A are assembled as large oligomers. More variability in the degree of oligomerization was observed with recombinant human SP-A than with natural canine SP-A. As shown by collagenase digestion, the full assembly of protein subunits was dependent on an intact collagen-like domain. The cysteines in the noncollagen domain of SP-A form intrachain bonds between residues 135-226 and 204-218. The circular dichroism spectra of both recombinant and natural SP-A were consistent with the presence of a collagen-like triple helix. As determined by the change in ellipticity at 205 nm, the thermal transition temperatures of canine, natural human, and recombinant SP-A were 51.5, 52.3, and 42.0 degrees C, respectively. These results suggest differences in the assembly and stability of the natural and recombinant proteins."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajplung.1989.257.6.l421"xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajplung.1989.257.6.l421"xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Schilling J."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Schilling J."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "White R.T."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "White R.T."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Lau K."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Lau K."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Haagsman H.P."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Haagsman H.P."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Clements J.A."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Clements J.A."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Benson B.J."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Benson B.J."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Hawgood S."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Hawgood S."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Golden J."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/author | "Golden J."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |
| http://purl.uniprot.org/citations/2610270 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |