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http://purl.uniprot.org/citations/26109584http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/26109584http://www.w3.org/2000/01/rdf-schema#comment"

Aims

Excitation-contraction coupling in cardiomyocytes requires the proper targeting and retention of membrane proteins to unique domains by adaptor proteins like ankyrin-B. While ankyrin-B has been shown to interact with a variety of membrane and structural proteins located at different subcellular domains in cardiomyocytes, what regulates the specificity of ankyrin-B for particular interacting proteins remains elusive.

Methods and results

Here, we report the identification of two novel ankyrin-B isoforms AnkB-188 and AnkB-212 in human, rat, and mouse hearts. Novel cDNAs for both isoforms were isolated by long-range PCR of reverse-transcribed mRNA isolated from human ventricular tissue. The isoforms can be discriminated based on their function and subcellular distribution in cardiomyocytes. Heterologous overexpression of AnkB-188 increases sodium-calcium exchanger (NCX) membrane expression and current, while selective knockdown of AnkB-188 in cardiomyocytes reduces NCX expression and localization in addition to causing irregular contraction rhythms. Using an isoform-specific antibody, we demonstrate that the expression of AnkB-212 is restricted to striated muscles and is localized to the M-line of cardiomyocytes by interacting with obscurin. Selective knockdown of AnkB-212 significantly attenuates the expression of endogenous ankyrin-B at the M-line but does not disrupt NCX expression at transverse tubules in cardiomyocytes.

Conclusion

The identification and characterization of two functionally distinct ankyrin-B isoforms in heart provide compelling evidence that alternative splicing of the ANK2 gene regulates the fidelity of ankyrin-B interactions with proteins."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.org/dc/terms/identifier"doi:10.1093/cvr/cvv184"xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/author"Hu H."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/author"Luo J."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/author"Wu H.C."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/author"Cunha S.R."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/author"Subramaniam J."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/author"Hashmi S.S."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/author"Yamankurt G."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/name"Cardiovasc Res"xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/pages"466-477"xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/title"Identification and characterization of two ankyrin-B isoforms in mammalian heart."xsd:string
http://purl.uniprot.org/citations/26109584http://purl.uniprot.org/core/volume"107"xsd:string
http://purl.uniprot.org/citations/26109584http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/26109584
http://purl.uniprot.org/citations/26109584http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/26109584
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http://purl.uniprot.org/uniprot/#_A0A571BDG9-mappedCitation-26109584http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26109584
http://purl.uniprot.org/uniprot/#_A0A668KLB0-mappedCitation-26109584http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26109584
http://purl.uniprot.org/uniprot/#_P97582-mappedCitation-26109584http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26109584
http://purl.uniprot.org/uniprot/#_Q8CB85-mappedCitation-26109584http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26109584
http://purl.uniprot.org/uniprot/#_S4R2T7-mappedCitation-26109584http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26109584
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