| http://purl.uniprot.org/citations/26133970 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26133970 | http://www.w3.org/2000/01/rdf-schema#comment | "Botanical herbs are consumed globally not only as an essential diet but also as medicines or as functional/recreational food supplements. The extract of the Apocynum venetum leaves (AVLE), also known as Luobuma, exerts its antihypertensive effect via dilating the blood vessels in an endothelium- and concentration-dependent manner with optimal effect seen at as low as 10 µg/mL. A commercial Luoboma "antihypertensive tea" is available commercially in the western province of China. The present study seeks to investigate the underlying cellular mechanisms of the nitric oxide (NO)-releasing property of AVLE in rat aortas and human umbilical vein endothelial cells (HUVECs). Endothelium-dependent relaxation induced by AVLE was assessed in organ chambers in the presence or absence of polyethyleneglycol catalase (PP2, 20 µM; inhibitor of Src kinase), wortmannin (30 nM) and LY294002 (20 µM; PI3 (phosphatidylinositol3)-Kinase inhibitor), N(G)-nitro-L-arginine (L-NAME, 100 µM; endothelial NO synthase inhibitor (eNOS)) and ODQ (1 µM; soluble guanylyl cyclase inhibitor). Total nitrite and nitrate (NOx) level and protein expression of p-Akt and p-eNOS were measured. AVLE-induced endothelium-dependent relaxation was reduced by PP2, wortmannin and LY294002 and abolished by L-NAME and ODQ. AVLE significantly increased total NOx level in rat aortas and in HUVECs compared to control. It also instigated phosphorylation of Akt and eNOS in cultured HUVECs in a concentration-dependent manner and this was markedly suppressed by PP2, wortmannin and LY294002. AVLE also inhibited superoxide generated from both NADPH oxidase and xanthine/xanthine oxidase system. Taken together, AVLE causes endothelium-dependent NO mediated relaxations of rat aortas through Src/PI3K/Akt dependent NO signalling pathway and possesses superoxide scavenging activity."xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.org/dc/terms/identifier | "doi:10.3390/nu7075220"xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/author | "Mustafa M.R."xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/author | "Murugan D."xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/author | "Lau Y.S."xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/author | "Kwan C.Y."xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/author | "Ling W.C."xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/name | "Nutrients"xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/pages | "5239-5253"xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/title | "Endothelium-Dependent Relaxation Effect of Apocynum venetum Leaf Extract via Src/PI3K/Akt Signalling Pathway."xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/26133970 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26133970 |
| http://purl.uniprot.org/citations/26133970 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26133970 |
| http://purl.uniprot.org/uniprot/#_P47196-mappedCitation-26133970 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26133970 |
| http://purl.uniprot.org/uniprot/P47196 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/26133970 |