| http://purl.uniprot.org/citations/26149094 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26149094 | http://www.w3.org/2000/01/rdf-schema#comment | "αA- and αB-crystallins are small heat shock proteins that bind thermodynamically destabilized proteins thereby inhibiting their aggregation. Highly expressed in the mammalian lens, the α-crystallins have been postulated to play a critical role in the maintenance of lens optical properties by sequestering age-damaged proteins prone to aggregation as well as through a multitude of roles in lens epithelial cells. Here, we have examined the role of α-crystallins in the development of the vertebrate zebrafish lens. For this purpose, we have carried out morpholino-mediated knockdown of αA-, αBa- and αBb-crystallin and characterized the gross morphology of the lens. We observed lens abnormalities, including increased reflectance intensity, as a consequence of the interference with expression of these proteins. These abnormalities were less frequent in transgenic zebrafish embryos expressing rat αA-crystallin suggesting a specific role of α-crystallins in embryonic lens development. To extend and confirm these findings, we generated an αA-crystallin knockout zebrafish line. A more consistent and severe lens phenotype was evident in maternal/zygotic αA-crystallin mutants compared to those observed by morpholino knockdown. The penetrance of the lens phenotype was reduced by transgenic expression of rat αA-crystallin and its severity was attenuated by maternal αA-crystallin expression. These findings demonstrate that the role of α-crystallins in lens development is conserved from mammals to zebrafish and set the stage for using the embryonic lens as a model system to test mechanistic aspects of α-crystallin chaperone activity and to develop strategies to fine-tune protein-protein interactions in aging and cataracts."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.exer.2015.07.001"xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/author | "Chen W."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/author | "Wu S.Y."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/author | "Mishra S."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/author | "Zou P."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/author | "Koteiche H.A."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/author | "Mchaourab H.S."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/author | "Knapik E."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/author | "Levic D.S."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/name | "Exp Eye Res"xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/pages | "104-113"xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/title | "A conserved role of alphaA-crystallin in the development of the zebrafish embryonic lens."xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://purl.uniprot.org/core/volume | "138"xsd:string |
| http://purl.uniprot.org/citations/26149094 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26149094 |
| http://purl.uniprot.org/citations/26149094 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26149094 |
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| http://purl.uniprot.org/uniprot/#_Q8UUZ6-mappedCitation-26149094 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26149094 |
| http://purl.uniprot.org/uniprot/#_Q9PUR2-mappedCitation-26149094 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26149094 |
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