| http://purl.uniprot.org/citations/26160175 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26160175 | http://www.w3.org/2000/01/rdf-schema#comment | "Antibodies to the autoantigen transglutaminase 2 (TG2) are a hallmark of celiac disease. We have studied the interaction between TG2 and an anti-TG2 antibody (679-14-E06) derived from a single gut IgA plasma cell of a celiac disease patient. The antibody recognizes one of four identified epitopes targeted by antibodies of plasma cells of the disease lesion. The binding interface was identified by small angle x-ray scattering, ab initio and rigid body modeling using the known crystal structure of TG2 and the crystal structure of the antibody Fab fragment, which was solved at 2.4 Å resolution. The result was confirmed by testing binding of the antibody to TG2 mutants by ELISA and surface plasmon resonance. TG2 residues Arg-116 and His-134 were identified to be critical for binding of 679-14-E06 as well as other epitope 1 antibodies. In contrast, antibodies directed toward the two other main epitopes (epitopes 2 and 3) were not affected by these mutations. Molecular dynamics simulations suggest interactions of 679-14-E06 with the N-terminal domain of TG2 via the CDR2 and CDR3 loops of the heavy chain and the CDR2 loop of the light chain. In addition there were contacts of the framework 3 region of the heavy chain with the catalytic domain of TG2. The results provide an explanation for the biased usage of certain heavy and light chain gene segments by epitope 1-specific antibodies in celiac disease."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.669895"xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/author | "Andersen J.T."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/author | "Svergun D."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/author | "Sollid L.M."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/author | "Tuukkanen A."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/author | "Graewert M.A."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/author | "Iversen R."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/author | "Hnida K."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/pages | "21365-21375"xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/title | "Structural Basis for Antigen Recognition by Transglutaminase 2-specific Autoantibodies in Celiac Disease."xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://purl.uniprot.org/core/volume | "290"xsd:string |
| http://purl.uniprot.org/citations/26160175 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26160175 |
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