| http://purl.uniprot.org/citations/26162986 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26162986 | http://www.w3.org/2000/01/rdf-schema#comment | "Head-to-head gene pairs represent a unique feature of gene organization in eukaryotes, accounting for >10% of genes in the human genome. Identification and functional analysis of such gene pairs is only in its infancy. Recently, we identified PRR11 as a novel cancer-related gene that is implicated in cell cycle and lung cancer. Here we demonstrate that PRR11 is oriented in a head-to-head configuration with its neighboring gene, SKA2. 5'-RACE assay revealed that the intergenic spacer region between the two genes is <500 bp. Serial luciferase reporter assays demonstrated that a minimal 80-bp intergenic region functions as a core bidirectional promoter to drive basal transcription in both the PRR11 and SKA2 orientations. EMSA and ChIP assays demonstrated that NF-Y binds to and directly transactivates the PRR11-SKA2 bidirectional promoter. SiRNA-mediated NF-Y depletion significantly downregulated PRR11 and SKA2 expression. Expression of both PRR11 and SKA2 was significantly upregulated in lung cancer. Expression of the two genes was highly correlated with each other and with NF-Y expression. Remarkably, high expression of both PRR11 and SKA2 was associated with poorer prognosis in lung cancer patients compared with high expression of one gene or low expression of both genes. Knockdown of PRR11 and/or SKA2 remarkably reduced cell proliferation, migration, and invasion in lung cancer cells. Thus, the PRR11-SKA2 bidirectional transcription unit, which is a novel direct target of NF-Y, is essential for the accelerated proliferation and motility of lung cancer cells and may represent a potential target in the diagnosis and/or treatment of human lung cancer."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbagrm.2015.07.002"xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Liu Z."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Niu Y."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Song F."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Wang S."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Zhang C."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Xie M."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Weng H."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Long Y."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Ozaki T."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Cai W."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Du G."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Ai Q."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/author | "Bu Y."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/pages | "1133-1144"xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/title | "The gene pair PRR11 and SKA2 shares a NF-Y-regulated bidirectional promoter and contributes to lung cancer development."xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://purl.uniprot.org/core/volume | "1849"xsd:string |
| http://purl.uniprot.org/citations/26162986 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26162986 |