| http://purl.uniprot.org/citations/26169224 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26169224 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26169224 | http://www.w3.org/2000/01/rdf-schema#comment | "MuB is an ATP-dependent DNA-binding protein that regulates the activity of MuA transposase and delivers the target DNA for transposition of phage Mu. Mechanistic insight into MuB function is limited to its AAA+ ATPase module, which upon ATP binding assembles into helical filaments around the DNA. However, the structure and function of the flexible N-terminal domain (NTD) appended to the AAA+ module remains uncharacterized. Here we report the solution structure of MuB NTD determined by NMR spectroscopy. The structure reveals a compact domain formed by four α-helices connected by short loops, and confirms the presence of a helix-turn-helix motif. High structural similarity and sequence homology with λ repressor-like DNA-binding domains suggest a possible role of MuB NTD in DNA binding. We also demonstrate that the NTD directly mediates the ability of MuB to establish filament-filament interactions. These findings lead us to a model in which the NTD interacts with the AAA+ spirals and perhaps also with the DNA bound within the filament, favoring MuB polymerization and filament clustering. We propose that the MuB NTD-dependent filament interactions might be an effective mechanism to bridge distant DNA regions during Mu transposition."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jsb.2015.07.004"xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jsb.2015.07.004"xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Ramon-Maiques S."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Ramon-Maiques S."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Campos-Olivas R."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Campos-Olivas R."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Lopez-Mendez B."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Lopez-Mendez B."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Boskovic J."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Boskovic J."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Dramicanin M."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/author | "Dramicanin M."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/name | "J. Struct. Biol."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/name | "J. Struct. Biol."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/pages | "100-111"xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/pages | "100-111"xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/title | "The N-terminal domain of MuB protein has striking structural similarity to DNA-binding domains and mediates MuB filament-filament interactions."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/title | "The N-terminal domain of MuB protein has striking structural similarity to DNA-binding domains and mediates MuB filament-filament interactions."xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/volume | "191"xsd:string |
| http://purl.uniprot.org/citations/26169224 | http://purl.uniprot.org/core/volume | "191"xsd:string |