| http://purl.uniprot.org/citations/26184350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26184350 | http://www.w3.org/2000/01/rdf-schema#comment | "The aim of the present work was to clarify whether heterotrimeric P2X2/3 receptors have a fixed subunit stoichiometry consisting of one P2X2 and two P2X3 subunits as previously suggested, or a flexible stoichiometry containing also the inverse subunit composition. For this purpose we transfected HEK293 cells with P2X2 and P2X3 encoding cDNA at the ratios of 1:2 and 4:1, and analysed the biophysical and pharmacological properties of the generated receptors by means of the whole-cell patch-clamp technique. The concentration-response curves for the selective agonist α,β-meATP did not differ from each other under the two transfection ratios. However, co-expression of an inactive P2X2 mutant and the wild type P2X3 subunit and vice versa resulted in characteristic distortions of the α,β-meATP concentration-response relationships, depending on which subunit was expressed in excess, suggesting that HEK293 cells express mixtures of (P2X2)1/(P2X3)2 and (P2X2)2/(P2X3)1 receptors. Whereas the allosteric modulators H+ and Zn2+ failed to discriminate between the two possible heterotrimeric receptor variants, the α,β-meATP-induced responses were blocked more potently by the competitive antagonist A317491, when the P2X2 subunit was expressed in deficit of the P2X3 subunit. Furthermore, blue-native PAGE analysis of P2X2 and P2X3 subunits co-expressed in Xenopus laevis oocytes and HEK293 cells revealed that plasma membrane-bound P2X2/3 receptors appeared in two clearly distinct heterotrimeric complexes: a (P2X2-GFP)2/(P2X3)1 complex and a (P2X2-GFP)1/(P2X3)2 complex. These data strongly indicate that the stoichiometry of the heteromeric P2X2/3 receptor is not fixed, but determined in a permutational manner by the relative availability of P2X2 and P2X3 subunits."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.neuropharm.2015.07.008"xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Hausmann R."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Tang Y."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Schmid J."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Kowalski M."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Schmalzing G."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Illes P."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Rubini P."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Dopychai A."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/author | "Stephan G."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/name | "Neuropharmacology"xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/pages | "115-130"xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/title | "Flexible subunit stoichiometry of functional human P2X2/3 heteromeric receptors."xsd:string |
| http://purl.uniprot.org/citations/26184350 | http://purl.uniprot.org/core/volume | "99"xsd:string |
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