| http://purl.uniprot.org/citations/2620834 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2620834 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2620834 | http://www.w3.org/2000/01/rdf-schema#comment | "The N-2 strain of Acremonium chrysogenum accumulates the beta-lactam precursor tripeptide delta-(L-alpha-amino-adipoyl)-L-cysteinyl-D-valine and has no discernible activity for three of the cephalosporin C (Ce) biosynthetic enzymes. This phenotype is consistent with a mutation either within pcbC [the isopenicillin N synthetase (IPNS)-encoding gene] or in a pathway-regulator gene. To distinguish these possibilities we have cloned and sequenced pcbC from strain N-2. There is a single C----T mutation at nt 854 within the coding sequence, changing aa 285 from proline to leucine. An IPNS-specific monoclonal antibody recognises a catalytically inactive IPNS protein in extracts of N-2 cells. These findings suggest that strain N-2 carries a simple IPNS mutation and that IPNS or its biosynthetic product isopenicillin N is involved in regulation of the later stages of the Ce biosynthetic pathway."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(89)90493-9"xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(89)90493-9"xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "Saunders K."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "Saunders K."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "Harford S."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "Harford S."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "McQuade B.A."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "McQuade B.A."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "Ramsden M."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "Ramsden M."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "Turner M.K."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/author | "Turner M.K."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/pages | "267-273"xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/pages | "267-273"xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/title | "Characterization of a loss-of-function mutation in the isopenicillin N synthetase gene of Acremonium chrysogenum."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/title | "Characterization of a loss-of-function mutation in the isopenicillin N synthetase gene of Acremonium chrysogenum."xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/volume | "85"xsd:string |
| http://purl.uniprot.org/citations/2620834 | http://purl.uniprot.org/core/volume | "85"xsd:string |