| http://purl.uniprot.org/citations/26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/26227410 | http://www.w3.org/2000/01/rdf-schema#comment | "Sulfur-containing aroma compounds are key contributors to the flavour of a diverse range of foods and beverages, such as wine. The tropical fruit characters of Sauvignon Blanc wines are attributed to the presence of the aromatic thiols 3-mercaptohexan-1-ol (3-MH), its acetate ester 3-mercaptohexyl acetate (3-MHA), and 4-mercapto-4-methylpentan-2-one (4-MMP). These aromatic thiols are not detectable in grape juice to any significant extent but are released by yeast during alcoholic fermentation. While the processes involved in the release of 3-MH and 4-MMP from their cysteinylated precursors have been studied extensively, degradation pathways for glutathione S-conjugates (GSH-3-MH and GSH-4-MMP) have not. In this study, a candidate gene approach was taken, focusing on genes known to play a role in glutathione and glutathione-S-conjugate turnover in Saccharomyces cerevisiae. Our results confirm the role of Opt1p as the major transporter responsible for uptake of GSH-3-MH and GSH-4-MMP, and identify vacuolar Ecm38p as a key determinant of 3-MH release from GSH-3-MH. ECM38 was unimportant, on the other hand, for release of 4-MMP, and abolition of vacuolar biogenesis caused an increase in the amount of 4-MMP released. The alternative cytosolic glutathione degradation pathway was not involved in release of either thiol from their glutathionylated precursors. Finally, cycling of GSH-3-MH and/or its breakdown intermediates between the cytosol and the vacuole or extracellular space was implicated in modulation of 3-MH formation. Together, these results provide new targets for development of yeast strains that optimize release of these potent volatile sulfur compounds, and further our understanding of the processes involved in glutathione-S-conjugate turnover."xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00253-015-6833-5"xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://purl.uniprot.org/core/author | "Cordente A.G."xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://purl.uniprot.org/core/author | "Curtin C.D."xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://purl.uniprot.org/core/author | "Capone D.L."xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26227410 | http://purl.uniprot.org/core/name | "Appl Microbiol Biotechnol"xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://purl.uniprot.org/core/pages | "9709-9722"xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://purl.uniprot.org/core/title | "Unravelling glutathione conjugate catabolism in Saccharomyces cerevisiae: the role of glutathione/dipeptide transporters and vacuolar function in the release of volatile sulfur compounds 3-mercaptohexan-1-ol and 4-mercapto-4-methylpentan-2-one."xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://purl.uniprot.org/core/volume | "99"xsd:string |
| http://purl.uniprot.org/citations/26227410 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26227410 |
| http://purl.uniprot.org/citations/26227410 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26227410 |
| http://purl.uniprot.org/citations/26227410 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26227410 |
| http://purl.uniprot.org/citations/26227410 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26227410 |
| http://purl.uniprot.org/enzyme/7.6.2.3 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/26227410 |
| http://purl.uniprot.org/uniprot/#_P53871-mappedCitation-26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26227410 |
| http://purl.uniprot.org/uniprot/#_P40897-mappedCitation-26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26227410 |
| http://purl.uniprot.org/uniprot/#_P12868-mappedCitation-26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26227410 |
| http://purl.uniprot.org/uniprot/#_P32386-mappedCitation-26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26227410 |
| http://purl.uniprot.org/uniprot/#_Q05902-mappedCitation-26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26227410 |
| http://purl.uniprot.org/uniprot/#_P32901-mappedCitation-26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26227410 |
| http://purl.uniprot.org/uniprot/#_P43616-mappedCitation-26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26227410 |
| http://purl.uniprot.org/uniprot/#_P53049-mappedCitation-26227410 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26227410 |