| http://purl.uniprot.org/citations/26240340 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26240340 | http://www.w3.org/2000/01/rdf-schema#comment | "Ubiquitylation of histone H2B at lysine 120 (H2B-Ub) plays a critical role in transcriptional elongation, chromatin conformation, as well as the regulation of specific histone H3 methylations. Herein, we report a strategy for the site-specific chemical attachment of ubiquitin to preassembled nucleosomes. This allowed expedited structure-activity studies into how H2B-Ub regulates H3K79 methylation by the methyltransferase human Dot1. Through an alanine scan of the ubiquitin surface, we identified a functional hotspot on ubiquitin that is required for the stimulation of human Dot1 in vitro. Importantly, this result was validated in chromatin from isolated nuclei by using a synthetic biology strategy that allowed selective incorporation of the hotspot-deficient ubiquitin mutant into H2B. The ubiquitin hotspot additionally impacted the regulation of ySet1-mediated H3K4 methylation but was not required for H2B-Ub-induced impairment of chromatin fiber compaction. These data demonstrate the utility of applying chemical ligation technologies to preassembled chromatin and delineate the multifunctionality of ubiquitin as a histone posttranslational modification."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1504483112"xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/author | "Kim J."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/author | "Tang Z."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/author | "Muir T.W."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/author | "Jeon J."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/author | "Roeder R.G."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/author | "David Y."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/author | "Pollock S."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/author | "Holt M.T."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/pages | "10365-10370"xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/title | "Identification of a functional hotspot on ubiquitin required for stimulation of methyltransferase activity on chromatin."xsd:string |
| http://purl.uniprot.org/citations/26240340 | http://purl.uniprot.org/core/volume | "112"xsd:string |
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