| http://purl.uniprot.org/citations/26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26260794 | http://www.w3.org/2000/01/rdf-schema#comment | "Damaged mitochondria are eliminated through autophagy machinery. A cytosolic E3 ubiquitin ligase Parkin, a gene product mutated in familial Parkinsonism, is essential for this pathway. Recent progress has revealed that phosphorylation of both Parkin and ubiquitin at Ser(65) by PINK1 are crucial for activation and recruitment of Parkin to the damaged mitochondria. However, the mechanism by which phosphorylated ubiquitin associates with and activates phosphorylated Parkin E3 ligase activity remains largely unknown. Here, we analyze interactions between phosphorylated forms of both Parkin and ubiquitin at a spatial resolution of the amino acid residue by site-specific photo-crosslinking. We reveal that the in-between-RING (IBR) domain along with RING1 domain of Parkin preferentially binds to ubiquitin in a phosphorylation-dependent manner. Furthermore, another approach, the Fluoppi (fluorescent-based technology detecting protein-protein interaction) assay, also showed that pathogenic mutations in these domains blocked interactions with phosphomimetic ubiquitin in mammalian cells. Molecular modeling based on the site-specific photo-crosslinking interaction map combined with mass spectrometry strongly suggests that a novel binding mechanism between Parkin and ubiquitin leads to a Parkin conformational change with subsequent activation of Parkin E3 ligase activity."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.671446"xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/author | "Tanaka K."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/author | "Yamano K."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/author | "Matsuda N."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/author | "Saeki Y."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/author | "Hirokawa T."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/author | "Koyano F."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/author | "Queliconi B.B."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/pages | "25199-25211"xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/title | "Site-specific Interaction Mapping of Phosphorylated Ubiquitin to Uncover Parkin Activation."xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://purl.uniprot.org/core/volume | "290"xsd:string |
| http://purl.uniprot.org/citations/26260794 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26260794 |
| http://purl.uniprot.org/citations/26260794 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26260794 |
| http://purl.uniprot.org/uniprot/#_D3JZW5-mappedCitation-26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26260794 |
| http://purl.uniprot.org/uniprot/#_D3K2X1-mappedCitation-26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26260794 |
| http://purl.uniprot.org/uniprot/#_D3K2X2-mappedCitation-26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26260794 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z446-mappedCitation-26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26260794 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z447-mappedCitation-26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26260794 |
| http://purl.uniprot.org/uniprot/#_A0A1W5XGD7-mappedCitation-26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26260794 |
| http://purl.uniprot.org/uniprot/#_A0A1W5XGE1-mappedCitation-26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26260794 |
| http://purl.uniprot.org/uniprot/#_A0A1W5XGE7-mappedCitation-26260794 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26260794 |