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http://purl.uniprot.org/citations/26302771http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/26302771http://www.w3.org/2000/01/rdf-schema#comment"

Background

In this work, we investigated the functional role of microRNA 137 (miR-137) in regulating osteosarcoma both in vitro and in vivo.

Methods

Quantitative RT-PCR was used to examine the gene expressions of miR-137 in osteosarcoma cell lines and osteosarcoma tumors. 143B and Saos-2 cells were infected with lentivirus expressing miR-137 mimics (miR-137-mimic) to ectopically upregulate miR-137. In vitro cancer proliferation and migration were examined by MTT assay and transwell assay, respectively. Viral infected Saos-2 cells were also subcutaneously inoculated into null mice to evaluate the effect of miR-137 upregulation on in vivo tumor growth. The interaction between miR-137 and its downstream target, FXYD6, was evaluated by dual-luciferase reporter assay and quantitative real-time PCR. FXYD6 was then subsequently upregulated in osteosarcoma cells to evaluate its effect on miR-137 regulation in osteosarcoma.

Results

We found that miR-137 was significantly downregulated in both osteosarcoma cell lines and osteosarcoma tumors. Lentiviral infection of miR-137-mimic upregulated miR-137 gene expression, reduced in vitro proliferation and migration and inhibited in vivo osteosarcoma tumor growth. FXYD6 was verified to be directly interacting with miR-137, and its subsequent upregulation reversed the inhibitory effect of miR-137 upregulation in osteosarcoma.

Conclusion

We revealed novel functional role of miR-137 in osteosarcoma regulation, likely through FXYD6 binding."xsd:string
http://purl.uniprot.org/citations/26302771http://purl.org/dc/terms/identifier"doi:10.3109/1061186x.2015.1057149"xsd:string
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/author"Wang Y.X."xsd:string
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/author"Zhang H.Y."xsd:string
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/author"Wang W.B."xsd:string
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/author"Li Z.M."xsd:string
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/name"J Drug Target"xsd:string
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/pages"102-110"xsd:string
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/title"MicroRNA-137 is downregulated in human osteosarcoma and regulates cell proliferation and migration through targeting FXYD6."xsd:string
http://purl.uniprot.org/citations/26302771http://purl.uniprot.org/core/volume"24"xsd:string
http://purl.uniprot.org/citations/26302771http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/26302771
http://purl.uniprot.org/citations/26302771http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/26302771
http://purl.uniprot.org/uniprot/#_J3QKN1-mappedCitation-26302771http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26302771
http://purl.uniprot.org/uniprot/#_Q9H0Q3-mappedCitation-26302771http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26302771
http://purl.uniprot.org/uniprot/Q9H0Q3http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/26302771
http://purl.uniprot.org/uniprot/J3QKN1http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/26302771