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http://purl.uniprot.org/citations/26305116http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Context

The antidiabetic drug metformin exhibits antiproliferative and pro-apoptotic effects in various cells, suggesting its potential to treat a variety of malignant and non-malignant hyperplastic diseases. Clinical studies indicate that psoriasis patients with metformin treatment have a better response than those without metformin.

Objective

The present study evaluates the antiproliferative activity and anti-inflammatory responses of metformin in human keratinocytes in vitro and explores the underlying mechanisms.

Materials and methods

HaCaT cells were incubated with metformin at 0, 25, 50, and 100 mM for 48 h. Antiproliferative activity was evaluated by MTT and apoptotic response was examined by flow cytometry. ELISA was used to detect IL-6, TNF-α, and VEGF protein expression. Western blot was used to investigate the expression of the mammalian target of rapamycin (mTOR) and its downstream effectors p70 ribosomal S6 kinase (p70S6K).

Results

The survival rates of HaCaT cells treated with metformin at 50 mM were reduced to 75.6, 59.4, and 30.3% at 24, 48, and 72 h, respectively. The number of apoptotic HaCaT cells was significantly increased at 50 mM metformin after 48 h treatment. Metformin can exert an anti-inflammatory effect by direct inhibition of IL-6, TNF-α, and VEGF. Metformin at 50 mM significantly reduced the phosphorylation of mTOR and p70S6K, by 49.0 and 62.1%, respectively.

Discussion and conclusion

Metformin treatment significantly inhibited proliferation and proinflammatory responses in HaCaT cells by a mechanism associated with inhibition of the mTOR signaling pathway. The results indicate that metformin may be used as a potential therapeutic agent for psoriasis."xsd:string
http://purl.uniprot.org/citations/26305116http://purl.org/dc/terms/identifier"doi:10.3109/13880209.2015.1057652"xsd:string
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/author"Liu Y."xsd:string
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/author"Ma W."xsd:string
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/author"Sun Q."xsd:string
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/author"Yang F."xsd:string
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/name"Pharm Biol"xsd:string
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/pages"1173-1178"xsd:string
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/title"Metformin inhibits proliferation and proinflammatory cytokines of human keratinocytes in vitro via mTOR-signaling pathway."xsd:string
http://purl.uniprot.org/citations/26305116http://purl.uniprot.org/core/volume"54"xsd:string
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