| http://purl.uniprot.org/citations/26405040 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26405040 | http://www.w3.org/2000/01/rdf-schema#comment | "The RAVE complex (regulator of the H(+)-ATPase of vacuolar and endosomal membranes) is required for biosynthetic assembly and glucose-stimulated reassembly of the yeast vacuolar H(+)-ATPase (V-ATPase). Yeast RAVE contains three subunits: Rav1, Rav2, and Skp1. Rav1 is the largest subunit, and it binds Rav2 and Skp1 of RAVE; the E, G, and C subunits of the V-ATPase peripheral V1 sector; and Vph1 of the membrane Vo sector. We identified Rav1 regions required for interaction with its binding partners through deletion analysis, co-immunoprecipitation, two-hybrid assay, and pulldown assays with expressed proteins. We find that Skp1 binding requires sequences near the C terminus of Rav1, V1 subunits E and C bind to a conserved region in the C-terminal half of Rav1, and the cytosolic domain of Vph1 binds near the junction of the Rav1 N- and C-terminal halves. In contrast, Rav2 binds to the N-terminal domain of Rav1, which can be modeled as a double β-propeller. Only the V1 C subunit binds to both Rav1 and Rav2. Using GFP-tagged RAVE subunits in vivo, we demonstrate glucose-dependent association of RAVE with the vacuolar membrane, consistent with its role in glucose-dependent V-ATPase assembly. It is known that V1 subunit C localizes to the V1-Vo interface in assembled V-ATPase complexes and is important in regulated disassembly of V-ATPases. We propose that RAVE cycles between cytosol and vacuolar membrane in a glucose-dependent manner, positioning V1 and V0 subcomplexes and orienting the V1 C subunit to promote assembly."xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.667634"xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/author | "Kane P.M."xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/author | "Tarsio M."xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/author | "Smardon A.M."xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/author | "Diakov T.T."xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/author | "Nasab N.D."xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/pages | "27511-27523"xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/title | "Molecular Interactions and Cellular Itinerary of the Yeast RAVE (Regulator of the H+-ATPase of Vacuolar and Endosomal Membranes) Complex."xsd:string |
| http://purl.uniprot.org/citations/26405040 | http://purl.uniprot.org/core/volume | "290"xsd:string |
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