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| http://purl.uniprot.org/citations/26443863 | http://www.w3.org/2000/01/rdf-schema#comment | "Phosphatidylethanolamine (PE) in the yeast Saccharomyces cerevisiae is synthesized through decarboxylation of phosphatidylserine (PS), catalysed by PS decarboxylase 1 (Psd1p) and 2 (Psd2p) and the cytidine 5'-diphosphate (CDP)-ethanolamine (CDP-Etn) pathway. PSD1 null (psd1Δ) and PSD2 null (psd2Δ) mutants are viable in a synthetic minimal medium, but a psd1Δ psd2Δ double mutant exhibits Etn auxotrophy, which is incorporated into PE through the CDP-Etn pathway. We have previously shown that psd1Δ is synthetic lethal with deletion of VID22 (vid22Δ) [Kuroda et al. (2011) Mol. Microbiol. 80: , 248-265]. In the present study, we found that vid22Δ mutant exhibits Etn auxotrophy under PSD1-depressed conditions. Deletion of VID22 in wild-type and PSD1-depressed cells caused partial defects in PE formation through decarboxylation of PS. The enzyme activity of PS decarboxylase in an extract of vid22Δ cells was ∼70% of that in wild-type cells and similar to that in psd2Δ cells and the PS decarboxylase activity remaining in the PSD1-depressed cells became almost negligible with deletion of VID22. Thus, the vid22Δ mutation was suggested to cause a defect in the Psd2p activity. Furthermore, vid22Δ cells were shown to be defective in expression of the PSD2 gene tagged with 6×HA, the defect being ameliorated by replacement of the native promoter of the PSD2 gene with a CYC1 promoter. In addition, an α-galactosidase reporter assay revealed that the activity of the promoter of the PSD2 gene in vid22Δ cells was ∼5% of that in wild-type cells. These results showed that VID22 is required for transcriptional activation of the PSD2 gene."xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20150884"xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/author | "Yamaguchi T."xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/author | "Miyoshi T."xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/author | "Tani M."xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/author | "Kuge O."xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/author | "Miyata N."xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/author | "Nakazono T."xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/pages | "319-328"xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/title | "VID22 is required for transcriptional activation of the PSD2 gene in the yeast Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/26443863 | http://purl.uniprot.org/core/volume | "472"xsd:string |
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