| http://purl.uniprot.org/citations/26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26451041 | http://www.w3.org/2000/01/rdf-schema#comment | "Tail-anchored (TA) proteins, defined as having a single transmembrane helix at their C terminus, are post-translationally targeted to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. In yeast, the handover of TA substrates is mediated by the heterotetrameric Get4/Get5 complex (Get4/5), which tethers the co-chaperone Sgt2 to the targeting factor, the Get3 ATPase. Binding of Get4/5 to Get3 is critical for efficient TA targeting; however, questions remain about the formation of the Get3·Get4/5 complex. Here we report crystal structures of a Get3·Get4/5 complex from Saccharomyces cerevisiae at 2.8 and 6.0 Å that reveal a novel interface between Get3 and Get4 dominated by electrostatic interactions. Kinetic and mutational analyses strongly suggest that these structures represent an on-pathway intermediate that rapidly assembles and then rearranges to the final Get3·Get4/5 complex. Furthermore, we provide evidence that the Get3·Get4/5 complex is dominated by a single Get4/5 heterotetramer bound to one monomer of a Get3 dimer, uncovering an intriguing asymmetry in the Get4/5 heterotetramer upon Get3 binding. Ultrafast diffusion-limited electrostatically driven Get3·Get4/5 association enables Get4/5 to rapidly sample and capture Get3 at different stages of the GET pathway."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.677328"xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/author | "Hess S."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/author | "Clemons W.M. Jr."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/author | "Shan S.O."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/author | "Rao M."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/author | "Chartron J.W."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/author | "Rome M.E."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/author | "Gristick H.B."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/pages | "30006-30017"xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/title | "Mechanism of Assembly of a Substrate Transfer Complex during Tail-anchored Protein Targeting."xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://purl.uniprot.org/core/volume | "290"xsd:string |
| http://purl.uniprot.org/citations/26451041 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26451041 |
| http://purl.uniprot.org/citations/26451041 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26451041 |
| http://purl.uniprot.org/uniprot/#_P63027-mappedCitation-26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26451041 |
| http://purl.uniprot.org/uniprot/#_Q13190-mappedCitation-26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26451041 |
| http://purl.uniprot.org/uniprot/#_P50402-mappedCitation-26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26451041 |
| http://purl.uniprot.org/uniprot/#_P51648-mappedCitation-26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26451041 |
| http://purl.uniprot.org/uniprot/#_Q12125-mappedCitation-26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26451041 |
| http://purl.uniprot.org/uniprot/#_Q12154-mappedCitation-26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26451041 |
| http://purl.uniprot.org/uniprot/#_Q12285-mappedCitation-26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26451041 |
| http://purl.uniprot.org/uniprot/#_Q16623-mappedCitation-26451041 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26451041 |