Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/26475744http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/26475744http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

Our previous study suggested that the coiled coil domain-containing 55 gene (CCDC55), also named as NSRP1 (nuclear speckle splicing regulatory protein 1 (NSRP1)), was encompassed in a haplotype block spanning over the serotonin transporter (5-HTT) gene in patients with schizophrenia (SCZ). However, the neurobiological function of CCDC55 gene remains unknown. This study aims to uncover the potential role of CCDC55 in SCZ-associated molecular pathways.

Experimental design

Using molecular cloning, sequencing and immune blotting to identify basic properties, yeast two-hybrid screening and glutathione S-transferase (GST) pull-down assay to test protein-protein interaction, and confocal laser scanning microscopy (CSLM) to show intracellular interaction of proteins.

Principal findings

(i) CCDC55 is expressed as a nuclear protein in human neuronal cells; (ii) Protein-protein interaction analyses showed CCDC55 physically interacted with Ran binding protein 9 (RanBP9) and disrupted in schizophrenia 1 (DISC1); (iii) CCDC55 and RanBP9 co-localized in the nucleus of human neuronal cells; (iv) CCDC55 also interacted with the cannabinoid receptor 1 (CNR1), and with the brain cannabinoid receptor-interacting protein 1a (CNRIP1a); (v) CNR1 activation in differentiated human neuronal cells resulted in an altered RanBP9 localization.

Conclusion

CCDC55 may be involved in a functional bridging between the CNR1 activation and the DISC1/RanBP9-associated pathways."xsd:string
http://purl.uniprot.org/citations/26475744http://purl.org/dc/terms/identifier"doi:10.1016/j.neuroscience.2015.10.012"xsd:string
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/author"Xie J."xsd:string
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/author"Vukojevic V."xsd:string
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/author"Leopardi R."xsd:string
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/author"Gizatullin R."xsd:string
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/name"Neuroscience"xsd:string
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/pages"723-730"xsd:string
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/title"The CCDC55 couples cannabinoid receptor CNR1 to a putative DISC1 schizophrenia pathway."xsd:string
http://purl.uniprot.org/citations/26475744http://purl.uniprot.org/core/volume"310"xsd:string
http://purl.uniprot.org/citations/26475744http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/26475744
http://purl.uniprot.org/citations/26475744http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/26475744
http://purl.uniprot.org/uniprot/#_A0A024QZ33-mappedCitation-26475744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/#_B7Z4B1-mappedCitation-26475744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/#_B2R9Y2-mappedCitation-26475744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/#_B7ZL27-mappedCitation-26475744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/#_Q05DD5-mappedCitation-26475744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/#_Q9H0G5-mappedCitation-26475744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/#_Q9P0E1-mappedCitation-26475744http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/Q9H0G5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/Q9P0E1http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/B7ZL27http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/26475744
http://purl.uniprot.org/uniprot/B2R9Y2http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/26475744
▶