| http://purl.uniprot.org/citations/26492619 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26492619 | http://www.w3.org/2000/01/rdf-schema#comment | "Podoplanin (PDPN) is a type-I transmembrane sialoglycoprotein that possesses a platelet aggregation-stimulating (PLAG) domain in the N-terminus. PLAG domain includes three tandem repeats of eight amino acids: PLAG1, PLAG2, and PLAG3. Among the three PLAG domains, O-glycan on Thr52 of PLAG3 is critical for binding with C-type lectin-like receptor-2 (CLEC-2) and is essential for platelet-aggregating activity of PDPN. In contrast, the glycosylation of Thr34 of PLAG1 of human PDPN remains to be clarified. Herein, we developed and characterized a novel anti-PDPN monoclonal antibody, LpMab-10, which targets PLAG1/2 domain. LpMab-10 detects endogenous PDPN of cancer cells and normal cells independently of glycosylation. The minimum epitope of LpMab-10 was identified as Glu33-Gly45 of PDPN using Western blot and flow cytometry. The Thr34 of PLAG1 is critical for LpMab-10 recognition, and O-glycan is not included in LpMab-10 epitope, indicating that Thr34 of PLAG1 is not O-glycosylated. In immunocytochemical and immunohistochemical analyses, LpMab-10 strongly detected PDPN-expressing tumor cells. By using monoclonal antibodies against different Ser/Thr, including epitopes of PDPN, it becomes possible to determine whether Ser/Thr residues of PDPN are O-glycosylated."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.org/dc/terms/identifier | "doi:10.1089/mab.2015.0018"xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Kato Y."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Fujii Y."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Goto K."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Honma R."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Nakamura T."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Takagi M."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Ogasawara S."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Oki H."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Hozumi Y."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/author | "Kaneko M.K."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/name | "Monoclon Antib Immunodiagn Immunother"xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/pages | "318-326"xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/title | "Development of Monoclonal Antibody LpMab-10 Recognizing Non-glycosylated PLAG1/2 Domain Including Thr34 of Human Podoplanin."xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://purl.uniprot.org/core/volume | "34"xsd:string |
| http://purl.uniprot.org/citations/26492619 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26492619 |
| http://purl.uniprot.org/citations/26492619 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26492619 |
| http://purl.uniprot.org/uniprot/#_A9XLN4-mappedCitation-26492619 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26492619 |
| http://purl.uniprot.org/uniprot/#_B2R968-mappedCitation-26492619 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26492619 |
| http://purl.uniprot.org/uniprot/#_Q6DJT9-mappedCitation-26492619 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26492619 |
| http://purl.uniprot.org/uniprot/B2R968 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/26492619 |