| http://purl.uniprot.org/citations/26503043 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26503043 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26503043 | http://www.w3.org/2000/01/rdf-schema#comment | "Bacteria and archaea generate adaptive immunity against phages and plasmids by integrating foreign DNA of specific 30-40-base-pair lengths into clustered regularly interspaced short palindromic repeat (CRISPR) loci as spacer segments. The universally conserved Cas1-Cas2 integrase complex catalyses spacer acquisition using a direct nucleophilic integration mechanism similar to retroviral integrases and transposases. How the Cas1-Cas2 complex selects foreign DNA substrates for integration remains unknown. Here we present X-ray crystal structures of the Escherichia coli Cas1-Cas2 complex bound to cognate 33-nucleotide protospacer DNA substrates. The protein complex creates a curved binding surface spanning the length of the DNA and splays the ends of the protospacer to allow each terminal nucleophilic 3'-OH to enter a channel leading into the Cas1 active sites. Phosphodiester backbone interactions between the protospacer and the proteins explain the sequence-nonspecific substrate selection observed in vivo. Our results uncover the structural basis for foreign DNA capture and the mechanism by which Cas1-Cas2 functions as a molecular ruler to dictate the sequence architecture of CRISPR loci."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature15760"xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature15760"xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Doudna J.A."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Doudna J.A."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Harrington L.B."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Harrington L.B."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Kranzusch P.J."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Kranzusch P.J."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Engelman A.N."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Engelman A.N."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Nunez J.K."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/author | "Nunez J.K."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/pages | "535-538"xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/pages | "535-538"xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/title | "Foreign DNA capture during CRISPR-Cas adaptive immunity."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/title | "Foreign DNA capture during CRISPR-Cas adaptive immunity."xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/volume | "527"xsd:string |
| http://purl.uniprot.org/citations/26503043 | http://purl.uniprot.org/core/volume | "527"xsd:string |