| http://purl.uniprot.org/citations/26505782 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26505782 | http://www.w3.org/2000/01/rdf-schema#comment | "Insulin-like growth factor-1 (IGF-1) is anabolic for muscle by enhancing the rate of mRNA translation via activation of AKT and subsequent activation of the mammalian target of rapamycin complex 1 (mTOR), thereby increasing cellular protein production. IGF-1 is also anabolic for bone, but whether the mTOR pathway plays a role in the rate of bone matrix protein production by osteoblasts is unknown. We hypothesized that anabolic stimuli such as mechanical loading and IGF-1 stimulate protein synthesis in osteoblasts via activation of the AKT-mTOR pathway. MC3T3-E1 osteoblasts were either or not subjected for 1 h to mechanical loading by pulsating fluid flow (PFF) or treated with or without human recombinant IGF-1 (1-100 ng/ml) for 0.5-6 h, to determine phosphorylation of AKT and p70S6K (downstream of mTOR) by Western blot. After 4 days of culture with or without the mTOR inhibitor rapamycin, total protein, DNA, and gene expression were quantified. IGF-1 (100 ng/ml) reduced IGF-1 gene expression, although PFF enhanced IGF-1 expression. IGF-1 did not affect collagen-I gene expression. IGF-1 dose-dependently enhanced AKT and p70S6K phosphorylation at 2 and 6 h. PFF enhanced phosphorylation of AKT and p70S6K already within 1 h. Both IGF-1 and PFF enhanced total protein per cell by ∼30%, but not in the presence of rapamycin. Our results show that IGF-1 and PFF activate mTOR, thereby stimulating the rate of mRNA translation in osteoblasts. The known anabolic effect of mechanical loading and IGF-1 on bone may thus be partly explained by mTOR-mediated enhanced protein synthesis in osteoblasts."xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.25228"xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/author | "Jaspers R.T."xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/author | "Bakker A.D."xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/author | "Klein-Nulend J."xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/author | "Gakes T."xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/author | "Hogervorst J.M."xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/author | "de Wit G.M."xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/name | "J Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/pages | "1283-1290"xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/title | "Mechanical Stimulation and IGF-1 Enhance mRNA Translation Rate in Osteoblasts Via Activation of the AKT-mTOR Pathway."xsd:string |
| http://purl.uniprot.org/citations/26505782 | http://purl.uniprot.org/core/volume | "231"xsd:string |
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