| http://purl.uniprot.org/citations/26517238 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26517238 | http://www.w3.org/2000/01/rdf-schema#comment | "RNase L is a regulated endoribonuclease that functions in the interferon antiviral response. Activation of RNase L by 2', 5'-oligoadenylates has been linked to apoptosis, autophagy and inflammation. Genetic studies have also suggested the possible involvement of the RNase L gene (RNASEL) on chromosome 1q25.3 in several types of cancer. Here we report that ablation of RNase L in human prostate cancer PC3 cells by CRISPR/Cas9 gene editing technology enhanced cell migration as determined both by transwell assays and scratch wound healing assays. In addition, RNase L knockdown by means of RNAi increased migration of PC3 and DU145 cells in response to either fibronectin or serum stimulation, as did homozygous disruption of the RNase L gene in mouse embryonic fibroblasts. Serum or fibronectin stimulation of focal adhesion kinase (FAK) autophosphorylation on tyrosine-397 was increased by either knockdown or ablation of RNase L. In contrast, a missense mutant RNase L (R667A) lacking catalytic activity failed to suppress cell migration in PC3 cells. However, a nuclease-inactive mutant mouse RNase L (W630A) was able to partially inhibit migration of mouse fibroblasts. Consistent with a role for the catalytic activity of RNase L, transfection of PC3 cells with the RNase L activator, 2', 5'-oligoadenylate, suppressed cell migration. RNase L knockdown in PC3 cells enhanced tumor growth and metastasis following implantation in the mouse prostate. Our results suggest that naturally occurring mutations in the RNase L gene might promote enhanced cell migration and metastasis."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.org/dc/terms/identifier | "doi:10.18632/oncotarget.6246"xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Li G."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Banerjee S."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Silverman R.H."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Gaughan C."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Weiss S.R."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Lindner D.J."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Parker Y."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/author | "Baskar D."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/name | "Oncotarget"xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/pages | "44360-44372"xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/title | "RNase L is a negative regulator of cell migration."xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/26517238 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26517238 |
| http://purl.uniprot.org/citations/26517238 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26517238 |
| http://purl.uniprot.org/uniprot/#_B2RQP1-mappedCitation-26517238 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26517238 |
| http://purl.uniprot.org/uniprot/#_P34152-mappedCitation-26517238 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26517238 |
| http://purl.uniprot.org/uniprot/#_Q1A3D9-mappedCitation-26517238 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26517238 |
| http://purl.uniprot.org/uniprot/#_Q05823-mappedCitation-26517238 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26517238 |
| http://purl.uniprot.org/uniprot/#_Q05921-mappedCitation-26517238 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26517238 |
| http://purl.uniprot.org/uniprot/#_Q05DT6-mappedCitation-26517238 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26517238 |