| http://purl.uniprot.org/citations/26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26529254 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein disulfide isomerase (PDI) has two distinct CGHC redox-active sites; however, the contribution of these sites during different physiologic reactions, including thrombosis, is unknown. Here, we evaluated the role of PDI and redox-active sites of PDI in thrombosis by generating mice with blood cells and vessel wall cells lacking PDI (Mx1-Cre Pdifl/fl mice) and transgenic mice harboring PDI that lacks a functional C-terminal CGHC motif [PDI(ss-oo) mice]. Both mouse models showed decreased fibrin deposition and platelet accumulation in laser-induced cremaster arteriole injury, and PDI(ss-oo) mice had attenuated platelet accumulation in FeCl3-induced mesenteric arterial injury. These defects were rescued by infusion of recombinant PDI containing only a functional C-terminal CGHC motif [PDI(oo-ss)]. PDI infusion restored fibrin formation, but not platelet accumulation, in eptifibatide-treated wild-type mice, suggesting a direct role of PDI in coagulation. In vitro aggregation of platelets from PDI(ss-oo) mice and PDI-null platelets was reduced; however, this defect was rescued by recombinant PDI(oo-ss). In human platelets, recombinant PDI(ss-oo) inhibited aggregation, while recombinant PDI(oo-ss) potentiated aggregation. Platelet secretion assays demonstrated that the C-terminal CGHC motif of PDI is important for P-selectin expression and ATP secretion through a non-αIIbβ3 substrate. In summary, our results indicate that the C-terminal CGHC motif of PDI is important for platelet function and coagulation."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.org/dc/terms/identifier | "doi:10.1172/jci80319"xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/author | "Wu Y."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/author | "Poncz M."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/author | "Essex D.W."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/author | "Hayes V.M."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/author | "Rauova L."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/name | "J Clin Invest"xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/pages | "4391-4406"xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/title | "The C-terminal CGHC motif of protein disulfide isomerase supports thrombosis."xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://purl.uniprot.org/core/volume | "125"xsd:string |
| http://purl.uniprot.org/citations/26529254 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26529254 |
| http://purl.uniprot.org/citations/26529254 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26529254 |
| http://purl.uniprot.org/uniprot/#_E9Q8G8-mappedCitation-26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26529254 |
| http://purl.uniprot.org/uniprot/#_F6Q404-mappedCitation-26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26529254 |
| http://purl.uniprot.org/uniprot/#_P97483-mappedCitation-26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26529254 |
| http://purl.uniprot.org/uniprot/#_Q3THC3-mappedCitation-26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26529254 |
| http://purl.uniprot.org/uniprot/#_O54890-mappedCitation-26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26529254 |
| http://purl.uniprot.org/uniprot/#_Q3UDR2-mappedCitation-26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26529254 |
| http://purl.uniprot.org/uniprot/#_P09103-mappedCitation-26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26529254 |
| http://purl.uniprot.org/uniprot/#_Q3U738-mappedCitation-26529254 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26529254 |