Results

RDF/XMLNTriplesTurtleShow queryShare
SubjectPredicateObject
http://purl.uniprot.org/citations/26567794http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/26567794http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

Ciliary neurotrophic factor (CNTF) was recently shown to augment cone function in CNGB3 mutant achromat dogs. However, testing CNTF-releasing implant in human CNGB3 achromats failed to show benefit. We evaluated the effects of CNTF protein on the retinal function in an additional achromatopsia model, the CNGB3-/- mouse.

Methods

Fifty-nine CNGB3-/-mice (postnatal day [PD] ± SD = 30 ± 7) received a unilateral intravitreal injection of 1 or 2 μg CNTF protein, and 15 wild-type (WT) mice (PD = 34 ± 3) received 1 μg CNTF. Retinal function was evaluated by flash ERG and photopic flicker ERG (fERG) at 7 and 14 days after treatment.

Results

Seven days post CNTF, the photopic b-wave Vmax was significantly increased in CNGB3-/-mice (P < 0.01), whereas it was reduced in WT mice (P < 0.05). Ciliary neurotrophic factor significantly increased the amplitude of photopic fERG and the photopic oscillatory potentials (OPs) in CNGB3-/-mice. Ciliary neurotrophic factor did not alter the scotopic a-wave in either CNGB3-/- or WT mice, but it increased the scotopic b-wave k (P < 0.01) in CNGB3-/-mice, indicating diminished scotopic sensitivity, and reduced the scotopic b-wave Vmax in WT mice (P < 0.05). No difference was found in ERG parameters between 1 or 2 μg CNTF. Fourteen days after CNTF injection the ERG changes in CNGB3-/-mice were lost.

Conclusions

Intravitreal bolus CNTF protein caused a small and transient improvement of cone-mediated function in CNGB3-/-mice, whereas it reduced rod-mediated function. The increase in photopic OPs and the lack of changes in scotopic a-wave suggest a CNTF effect on the inner retina."xsd:string
http://purl.uniprot.org/citations/26567794http://purl.org/dc/terms/identifier"doi:10.1167/iovs.15-16866"xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/author"Wen R."xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/author"Sieving P.A."xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/author"Bush R.A."xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/author"Vijayasarathy C."xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/author"Wei L.L."xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/author"Marangoni D."xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/date"2015"xsd:gYear
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/name"Invest Ophthalmol Vis Sci"xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/pages"6810-6822"xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/title"Intravitreal Ciliary Neurotrophic Factor Transiently Improves Cone-Mediated Function in a CNGB3-/- Mouse Model of Achromatopsia."xsd:string
http://purl.uniprot.org/citations/26567794http://purl.uniprot.org/core/volume"56"xsd:string
http://purl.uniprot.org/citations/26567794http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/26567794
http://purl.uniprot.org/citations/26567794http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/26567794
http://purl.uniprot.org/uniprot/#_Q9JJZ9-mappedCitation-26567794http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/26567794
http://purl.uniprot.org/uniprot/Q9JJZ9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/26567794