| http://purl.uniprot.org/citations/26571400 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26571400 | http://www.w3.org/2000/01/rdf-schema#comment | "Strategies aimed at mimicking or enhancing the action of the incretin hormone glucagon-like peptide 1 (GLP-1) therapeutically improve glucose-stimulated insulin secretion (GSIS); however, it is not clear whether GLP-1 directly drives insulin secretion in pancreatic islets. Here, we examined the mechanisms by which GLP-1 stimulates insulin secretion in mouse and human islets. We found that GLP-1 enhances GSIS at a half-maximal effective concentration of 0.4 pM. Moreover, we determined that GLP-1 activates PLC, which increases submembrane diacylglycerol and thereby activates PKC, resulting in membrane depolarization and increased action potential firing and subsequent stimulation of insulin secretion. The depolarizing effect of GLP-1 on electrical activity was mimicked by the PKC activator PMA, occurred without activation of PKA, and persisted in the presence of PKA inhibitors, the KATP channel blocker tolbutamide, and the L-type Ca(2+) channel blocker isradipine; however, depolarization was abolished by lowering extracellular Na(+). The PKC-dependent effect of GLP-1 on membrane potential and electrical activity was mediated by activation of Na(+)-permeable TRPM4 and TRPM5 channels by mobilization of intracellular Ca(2+) from thapsigargin-sensitive Ca(2+) stores. Concordantly, GLP-1 effects were negligible in Trpm4 or Trpm5 KO islets. These data provide important insight into the therapeutic action of GLP-1 and suggest that circulating levels of this hormone directly stimulate insulin secretion by β cells."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.org/dc/terms/identifier | "doi:10.1172/jci81975"xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Chibalina M.V."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Johnson P.R."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Ladds G."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Cha C.Y."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Katsura M."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Rorsman P."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Kaku K."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Zaccolo M."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Salehi A."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Galione A."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Nikolaev V.O."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Ramracheya R."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Tarasov A.I."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Vergari E."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Hastoy B."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Vennekens R."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Weston C."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Sones W.R."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Shigeto M."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Gorelik J."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Reinbothe T."xsd:string |
| http://purl.uniprot.org/citations/26571400 | http://purl.uniprot.org/core/author | "Philippaert K."xsd:string |