| http://purl.uniprot.org/citations/26582731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26582731 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26582731 | http://www.w3.org/2000/01/rdf-schema#comment | "The Mas-related G protein-coupled receptor X1 (MrgprX1) is a human seven transmembrane-domain protein with a putative role in nociception and pruritus. This receptor is expressed in dorsal root ganglion neurons and is activated by a variety of endogenous peptides, including bovine adrenal medulla peptide (BAM) and γ2-melanocyte-stimulating hormone (γ2-MSH). In the present work, we study how naturally occurring missense mutations alter the activity of MrgprX1. To characterize selected receptor variants, we initially used the endogenous peptide ligand BAM8-22. In addition, we generated and characterized a panel of novel recombinant and synthetic peptide ligands. Our studies identified a mutation in the second intracellular loop of MrgprX1, R131S, that causes a decrease in both ligand-mediated and constitutive signaling. Another mutation in this region, H133R, results in a gain of function phenotype reflected by an increase in ligand-mediated signaling. Using epitope-tagged variants, we determined that the alterations in basal and ligand-mediated signaling were not explained by changes in receptor expression levels. Our results demonstrate that naturally occurring mutations can alter the pharmacology of MrgprX1. This study provides a theoretical basis for exploring whether MrgprX1 variability underlies differences in somatosensation within human populations."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.org/dc/terms/identifier | "doi:10.1124/jpet.115.227058"xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.org/dc/terms/identifier | "doi:10.1124/jpet.115.227058"xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Kumar K."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Kumar K."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Doyle J.R."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Doyle J.R."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Beinborn M."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Beinborn M."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Kopin A.S."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Kopin A.S."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Raman V.S."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Raman V.S."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Heller D."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/author | "Heller D."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/name | "J. Pharmacol. Exp. Ther."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/name | "J. Pharmacol. Exp. Ther."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/pages | "276-283"xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/pages | "276-283"xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/title | "Novel probes establish mas-related G protein-coupled receptor X1 variants as receptors with loss or gain of function."xsd:string |
| http://purl.uniprot.org/citations/26582731 | http://purl.uniprot.org/core/title | "Novel probes establish mas-related G protein-coupled receptor X1 variants as receptors with loss or gain of function."xsd:string |