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http://purl.uniprot.org/citations/2659345http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2659345http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Citation
http://purl.uniprot.org/citations/2659345http://www.w3.org/2000/01/rdf-schema#comment"The enzyme GDP mannose:dolichyl-phosphate O-beta-D-mannosyltransferase (GDP-Man:DolP mannosyltransferase) catalyzing the reaction: GDP-man + DolP in equilibrium DolP-Man + GDP has been purified from Saccharomyces cerevisiae to homogeneity. The purification was achieved using a combination of column chromatographic methods with preparative gel electrophoresis. The enzyme has an apparent molecular mass of 30 kDa on SDS/polyacrylamide gels. Enzymatic activity could be correlated directly with this band. Antibodies against the transferase were raised in rabbits. The immune serum obtained removed enzymatic activity from a detergent extract of yeast membranes and reacted specifically with the 30-kDa band on immunoblots. Experiments addressing the orientation of this enzyme in the endoplasmic reticulum membrane are presented by using selective trypsin and N-ethylmaleimide treatment."xsd:string
http://purl.uniprot.org/citations/2659345http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1989.tb14774.x"xsd:string
http://purl.uniprot.org/citations/2659345http://purl.uniprot.org/core/author"Haselbeck A."xsd:string
http://purl.uniprot.org/citations/2659345http://purl.uniprot.org/core/date"1989"xsd:gYear
http://purl.uniprot.org/citations/2659345http://purl.uniprot.org/core/name"Eur J Biochem"xsd:string
http://purl.uniprot.org/citations/2659345http://purl.uniprot.org/core/pages"663-668"xsd:string
http://purl.uniprot.org/citations/2659345http://purl.uniprot.org/core/title"Purification of GDP mannose:dolichyl-phosphate O-beta-D-mannosyltransferase from Saccharomyces cerevisiae."xsd:string
http://purl.uniprot.org/citations/2659345http://purl.uniprot.org/core/volume"181"xsd:string
http://purl.uniprot.org/citations/2659345http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/2659345
http://purl.uniprot.org/citations/2659345http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/2659345
http://purl.uniprot.org/citations/2659345http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/2659345
http://purl.uniprot.org/citations/2659345http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/2659345
http://purl.uniprot.org/enzyme/2.4.1.83http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/2659345
http://purl.uniprot.org/uniprot/#_P14020-mappedCitation-2659345http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/2659345
http://purl.uniprot.org/uniprot/P14020http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/2659345