| http://purl.uniprot.org/citations/26598518 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26598518 | http://www.w3.org/2000/01/rdf-schema#comment | "We have previously reported that Ahnak-mediated TGFβ signaling leads to down-regulation of c-Myc expression. Here, we show that inhibition of Ahnak can promote generation of induced pluripotent stem cells (iPSC) via up-regulation of endogenous c-Myc. Consistent with the c-Myc inhibitory role of Ahnak, mouse embryonic fibroblasts from Ahnak-deficient mouse (Ahnak(-/-) MEF) show an increased level of c-Myc expression compared with wild type MEF. Generation of iPSC with just three of the four Yamanaka factors, Oct4, Sox2, and Klf4 (hereafter 3F), was significantly enhanced in Ahnak(-/-) MEF. Similar results were obtained when Ahnak-specific shRNA was applied to wild type MEF. Of note, expressionof Ahnak was significantly induced during the formation of embryoid bodies from embryonic stem cells, suggesting that Ahnak-mediated c-Myc inhibition is involved in embryoid body formation and the initial differentiation of pluripotent stem cells. The iPSC from 3F-infected Ahnak(-/-) MEF cells (Ahnak(-/-)-iPSC-3F) showed expression of all stem cell markers examined and the capability to form three primary germ layers. Moreover, injection of Ahnak(-/-)-iPSC-3F into athymic nude mice led to development of teratoma containing tissues from all three primary germ layers, indicating that iPSC from Ahnak(-/-) MEF are bona fide pluripotent stem cells. Taken together, these data provide evidence for a new role for Ahnak in cell fate determination during development and suggest that manipulation of Ahnak and the associated signaling pathway may provide a means to regulate iPSC generation."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.659276"xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/author | "Kim J."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/author | "Kim K.S."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/author | "Park C.H."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/author | "Kim J.'"xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/author | "Bae Y.S."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/author | "Lee S.A."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/author | "Lim H.J."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/author | "Lee M.R."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/pages | "752-761"xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/title | "Regulation of c-Myc Expression by Ahnak Promotes Induced Pluripotent Stem Cell Generation."xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/26598518 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26598518 |
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