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http://purl.uniprot.org/citations/26608316http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/26608316http://www.w3.org/2000/01/rdf-schema#comment"

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Mammalian prions are proteinaceous infectious agents composed of misfolded assemblies of the host-encoded, cellular prion protein (PrP). Physiologically, the N-terminal polybasic region of residues 23 to 31 of PrP has been shown to be involved in its endocytic trafficking and interactions with glycosaminoglycans or putative ectodomains of membrane-associated proteins. Several recent reports also describe this PrP region as important for the toxicity of mutant prion proteins and the efficiency of prion propagation, both in vitro and in vivo. The question remains as to whether the latter observations made with mouse PrP and mouse prions would be relevant to other PrP species/prion strain combinations given the dramatic impact on prion susceptibility of minimal amino acid substitutions and structural variations in PrP. Here, we report that transgenic mouse lines expressing ovine PrP with a deletion of residues 23 to 26 (KKRP) or mutated in this N-terminal region (KQHPH instead of KKRPK) exhibited a variable, strain-dependent susceptibility to prion infection with regard to the proportion of affected mice and disease tempo relative to findings in their wild-type counterparts. Deletion has no major effect on 127S scrapie prion pathogenesis, whereas mutation increased by almost 3-fold the survival time of the mice. Deletion marginally affected the incubation time of scrapie LA19K and ovine bovine spongiform encephalopathy (BSE) prions, whereas mutation caused apparent resistance to disease.

Importance

Recent reports suggested that the N-terminal polybasic region of the prion protein could be a therapeutic target to prevent prion propagation or toxic signaling associated with more common neurodegenerative diseases such as Alzheimer's disease. Mutating or deleting this region in ovine PrP completes the data previously obtained with the mouse protein by identifying the key amino acid residues involved."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.org/dc/terms/identifier"doi:10.1128/jvi.02805-15"xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Moudjou M."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Laude H."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Herzog L."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Paquet-Fifield S."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Castille J."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Passet B."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Andreoletti O."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Moazami-Goudarzi K."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Vilotte J.L."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Makhzami S."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Beringue V."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Khalife M."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Vilotte M."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Vilette D."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/author"Reine F."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/name"J Virol"xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/pages"1638-1646"xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/title"Mutated but Not Deleted Ovine PrP(C) N-Terminal Polybasic Region Strongly Interferes with Prion Propagation in Transgenic Mice."xsd:string
http://purl.uniprot.org/citations/26608316http://purl.uniprot.org/core/volume"90"xsd:string
http://purl.uniprot.org/citations/26608316http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/26608316
http://purl.uniprot.org/citations/26608316http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/26608316
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