| http://purl.uniprot.org/citations/26649771 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26649771 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26649771 | http://www.w3.org/2000/01/rdf-schema#comment | "Previously, using the Illumina HumanHT-12 microarray we found that β-defensin 131 (DEFB131), an antimicrobial peptide, is upregulated in the human prostate epithelial cell line RWPE-1 upon stimulation with lipoteichoic acid (LTA; a gram-positive bacterial component), than that in the untreated RWPE-1 cells. In the current study, we aimed to investigate the role of DEFB131 in RWPE-1 cells during bacterial infection. We examined the intracellular signaling pathways and nuclear responses in RWPE-1 cells that contribute to DEFB131 gene induction upon stimulation with LTA. Chromatin immunoprecipitation was performed to determine whether NF-κB directly binds to the DEFB131 promoter after LTA stimulation in RWPE-1 cells. We found that DEFB131 expression was induced by LTA stimulation through TLR2 and p38MAPK/NF-κB activation, which was evident in the phosphorylation of both p38MAPK and IκBα. We also found that SB203580 and Bay11-7082, inhibitors of p38MAPK and NF-κB, respectively, suppressed LTA-induced DEFB131 expression. The chromatin immunoprecipitation assay showed that NF-κB directly binds to the DEFB131 promoter, suggesting that NF-κB is a direct regulator, and is necessary for LTA-induced DEFB131 expression in RWPE-1 cells. Interestingly, with DEFB131 overexpression in RWPE-1 cells, the accumulation of mRNA and protein secretion of cytokines (IL-1α, IL-1β, IL-6, and IL-12α) and chemokines (CCL20, CCL22, and CXCL8) were significantly enhanced. In addition, DEFB131-transfected RWPE-1 cells markedly induced chemotactic activity in THP-1 monocytes. We concluded that DEFB131 induces cytokine and chemokine upregulation through the TLR2/NF-κB signaling pathway in RWPE-1 cells during bacterial infection and promotes an innate immune response."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0144776"xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0144776"xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Kim J.H."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Kim J.H."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Kim H.J."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Kim H.J."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Kim K.H."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Kim K.H."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Lee J."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Lee J."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Myung S.C."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/author | "Myung S.C."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/pages | "E0144776"xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/pages | "E0144776"xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/title | "Expression of Beta-Defensin 131 Promotes an Innate Immune Response in Human Prostate Epithelial Cells."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/title | "Expression of Beta-Defensin 131 Promotes an Innate Immune Response in Human Prostate Epithelial Cells."xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/26649771 | http://purl.uniprot.org/core/volume | "10"xsd:string |