| http://purl.uniprot.org/citations/26673105 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26673105 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26673105 | http://www.w3.org/2000/01/rdf-schema#comment | "Spliceosomal Prp38 proteins contain a conserved amino-terminal domain, but only higher eukaryotic orthologs also harbor a carboxy-terminal RS domain, a hallmark of splicing regulatory SR proteins. We show by crystal structure analysis that the amino-terminal domain of human Prp38 is organized around three pairs of antiparallel α-helices and lacks similarities to RNA-binding domains found in canonical SR proteins. Instead, yeast two-hybrid analyses suggest that the amino-terminal domain is a versatile protein-protein interaction hub that possibly binds 12 other spliceosomal proteins, most of which are recruited at the same stage as Prp38. By quantitative, alanine surface-scanning two-hybrid screens and biochemical analyses we delineated four distinct interfaces on the Prp38 amino-terminal domain. In vitro interaction assays using recombinant proteins showed that Prp38 can bind at least two proteins simultaneously via two different interfaces. Addition of excess Prp38 amino-terminal domain to in vitro splicing assays, but not of an interaction-deficient mutant, stalled splicing at a precatalytic stage. Our results show that human Prp38 is an unusual SR protein, whose amino-terminal domain is a multi-interface protein-protein interaction platform that might organize the relative positioning of other proteins during splicing."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.054296.115"xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.054296.115"xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Seeger M."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Seeger M."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Wahl M.C."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Wahl M.C."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Weber G."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Weber G."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Schuetze T."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Schuetze T."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Luehrmann R."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Luehrmann R."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Will C.L."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Will C.L."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Stelzl U."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Stelzl U."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Apelt L."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Apelt L."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Bartlick N."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Bartlick N."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Ulrich A.K."xsd:string |
| http://purl.uniprot.org/citations/26673105 | http://purl.uniprot.org/core/author | "Ulrich A.K."xsd:string |