| http://purl.uniprot.org/citations/26674602 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26674602 | http://www.w3.org/2000/01/rdf-schema#comment | "Hypokalemia (low serum potassium level) is a common electrolyte imbalance that can cause a defect in urinary concentrating ability, i.e., nephrogenic diabetes insipidus (NDI), but the molecular mechanism is unknown. We employed proteomic analysis of inner medullary collecting ducts (IMCD) from rats fed with a potassium-free diet for 1 day. IMCD protein quantification was performed by mass spectrometry using a label-free methodology. A total of 131 proteins, including the water channel AQP2, exhibited significant changes in abundance, most of which were decreased. Bioinformatic analysis revealed that many of the down-regulated proteins were associated with the biological processes of generation of precursor metabolites and energy, actin cytoskeleton organization, and cell-cell adhesion. Targeted LC-MS/MS and immunoblotting studies further confirmed the down regulation of 18 selected proteins. Electron microscopy showed autophagosomes/autophagolysosomes in the IMCD cells of rats deprived of potassium for only 1 day. An increased number of autophagosomes was also confirmed by immunofluorescence, demonstrating co-localization of LC3 and Lamp1 with AQP2 and several other down-regulated proteins in IMCD cells. AQP2 was also detected in autophagosomes in IMCD cells of potassium-deprived rats by immunogold electron microscopy. Thus, enhanced autophagic degradation of proteins, most notably including AQP2, is an early event in hypokalemia-induced NDI."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep18311"xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Hoffert J.D."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Pisitkun T."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Fenton R.A."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Chen S.H."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Khositseth S."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Saeed F."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Somparn P."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Thippamom N."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Uawithya P."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Charngkaew K."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/author | "Michael Payne D."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/date | "2015"xsd:gYear |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/pages | "18311"xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/title | "Autophagic degradation of aquaporin-2 is an early event in hypokalemia-induced nephrogenic diabetes insipidus."xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/26674602 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26674602 |
| http://purl.uniprot.org/citations/26674602 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26674602 |
| http://purl.uniprot.org/uniprot/#_P34080-mappedCitation-26674602 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26674602 |
| http://purl.uniprot.org/uniprot/P34080 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/26674602 |