| http://purl.uniprot.org/citations/26681692 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26681692 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26681692 | http://www.w3.org/2000/01/rdf-schema#comment | "Post-transcriptional modifications at the anticodon first (wobble) position of tRNA play critical roles in precise decoding of genetic codes. 5-carboxymethoxyuridine (cmo(5)U) and its methyl ester derivative 5-methoxycarbonylmethoxyuridine (mcmo(5)U) are modified nucleosides found at the anticodon wobble position in several tRNAs from Gram-negative bacteria. cmo(5)U and mcmo(5)U facilitate non-Watson-Crick base pairing with guanosine and pyrimidines at the third positions of codons, thereby expanding decoding capabilities. By mass spectrometric analyses of individual tRNAs and a shotgun approach of total RNA from Escherichia coli, we identified mcmo(5)U as a major modification in tRNA(Ala1), tRNA(Ser1), tRNA(Pro3) and tRNA(Thr4); by contrast, cmo(5)U was present primarily in tRNA(Leu3) and tRNA(Val1). In addition, we discovered 5-methoxycarbonylmethoxy-2'-O-methyluridine (mcmo(5)Um) as a novel but minor modification in tRNA(Ser1). Terminal methylation frequency of mcmo(5)U in tRNA(Pro3) was low (≈30%) in the early log phase of cell growth, gradually increased as growth proceeded and reached nearly 100% in late log and stationary phases. We identified CmoM (previously known as SmtA), an AdoMet-dependent methyltransferase that methylates cmo(5)U to form mcmo(5)U. A luciferase reporter assay based on a +1 frameshift construct revealed that terminal methylation of mcmo(5)U contributes to the decoding ability of tRNA(Ala1)."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkv1470"xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkv1470"xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/author | "Kimura S."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/author | "Kimura S."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/author | "Miyauchi K."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/author | "Miyauchi K."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/author | "Suzuki T."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/author | "Suzuki T."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/author | "Sakai Y."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/author | "Sakai Y."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/pages | "509-523"xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/pages | "509-523"xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/title | "Biogenesis and growth phase-dependent alteration of 5-methoxycarbonylmethoxyuridine in tRNA anticodons."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/title | "Biogenesis and growth phase-dependent alteration of 5-methoxycarbonylmethoxyuridine in tRNA anticodons."xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/volume | "44"xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://purl.uniprot.org/core/volume | "44"xsd:string |
| http://purl.uniprot.org/citations/26681692 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26681692 |
| http://purl.uniprot.org/citations/26681692 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26681692 |