| http://purl.uniprot.org/citations/26703466 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26703466 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26703466 | http://www.w3.org/2000/01/rdf-schema#comment | "GCAP1, a member of the neuronal calcium sensor subclass of the calmodulin superfamily, confers Ca(2+)-sensitive activation of retinal guanylyl cyclase 1 (RetGC1). We present NMR resonance assignments, residual dipolar coupling data, functional analysis, and a structural model of GCAP1 mutant (GCAP1(V77E)) in the Ca(2+)-free/Mg(2+)-bound state. NMR chemical shifts and residual dipolar coupling data reveal Ca(2+)-dependent differences for residues 170-174. An NMR-derived model of GCAP1(V77E) contains Mg(2+) bound at EF2 and looks similar to Ca(2+) saturated GCAP1 (root mean square deviations = 2.0 Å). Ca(2+)-dependent structural differences occur in the fourth EF-hand (EF4) and adjacent helical region (residues 164-174 called the Ca(2+) switch helix). Ca(2+)-induced shortening of the Ca(2+) switch helix changes solvent accessibility of Thr-171 and Leu-174 that affects the domain interface. Although the Ca(2+) switch helix is not part of the RetGC1 binding site, insertion of an extra Gly residue between Ser-173 and Leu-174 as well as deletion of Arg-172, Ser-173, or Leu-174 all caused a decrease in Ca(2+) binding affinity and abolished RetGC1 activation. We conclude that Ca(2+)-dependent conformational changes in the Ca(2+) switch helix are important for activating RetGC1 and provide further support for a Ca(2+)-myristoyl tug mechanism."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.696161"xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m115.696161"xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Lim S."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Lim S."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Ames J.B."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Ames J.B."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Dizhoor A.M."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Dizhoor A.M."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Olshevskaya E.V."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Olshevskaya E.V."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Peshenko I.V."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/author | "Peshenko I.V."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/pages | "4429-4441"xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/pages | "4429-4441"xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/title | "Structure of guanylyl cyclase activator protein 1 (GCAP1) mutant V77E in a Ca2+-free/Mg2+-bound activator state."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/title | "Structure of guanylyl cyclase activator protein 1 (GCAP1) mutant V77E in a Ca2+-free/Mg2+-bound activator state."xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/volume | "291"xsd:string |
| http://purl.uniprot.org/citations/26703466 | http://purl.uniprot.org/core/volume | "291"xsd:string |