| http://purl.uniprot.org/citations/26712002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26712002 | http://www.w3.org/2000/01/rdf-schema#comment | "Unique among the nuclear receptor superfamily, the glucocorticoid (GC) receptor (GR) can exert three distinct transcriptional regulatory functions on binding of a single natural (cortisol in human and corticosterone in mice) and synthetic [e.g., dexamethasone (Dex)] hormone. The molecular mechanisms underlying GC-induced positive GC response element [(+)GRE]-mediated activation of transcription are partially understood. In contrast, these mechanisms remain elusive for GC-induced evolutionary conserved inverted repeated negative GC response element (IR nGRE)-mediated direct transrepression and for tethered indirect transrepression that is mediated by DNA-bound NF-κB/activator protein 1 (AP1)/STAT3 activators and instrumental in GC-induced anti-inflammatory activity. We demonstrate here that SUMOylation of lysine K293 (mouse K310) located within an evolutionary conserved sequence in the human GR N-terminal domain allows the formation of a GR-small ubiquitin-related modifiers (SUMOs)-NCoR1/SMRT-HDAC3 repressing complex mandatory for GC-induced IR nGRE-mediated direct repression in vitro, but does not affect transactivation. Importantly, these results were validated in vivo: in K310R mutant mice and in mice ablated selectively for nuclear receptor corepressor 1 (NCoR1)/silencing mediator for retinoid or thyroid-hormone receptors (SMRT) corepressors in skin keratinocytes, Dex-induced direct repression and the formation of repressing complexes on IR nGREs were impaired, whereas transactivation was unaffected. In mice selectively ablated for histone deacetylase 3 (HDAC3) in skin keratinocytes, GC-induced direct repression, but not bindings of GR and of corepressors NCoR1/SMRT, was abolished, indicating that HDAC3 is instrumental in IR nGRE-mediated repression. Moreover, we demonstrate that the binding of HDAC3 to IR nGREs in vivo is mediated through interaction with SMRT/NCoR1. We also show that the GR ligand binding domain (LBD) is not required for SMRT-mediated repression, which can be mediated by a LBD-truncated GR, whereas it is mandatory for NCoR1-mediated repression through an interaction with K579 in the LBD."xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1522821113"xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://purl.uniprot.org/core/author | "Hua G."xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://purl.uniprot.org/core/author | "Chambon P."xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://purl.uniprot.org/core/author | "Paulen L."xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26712002 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://purl.uniprot.org/core/pages | "E626-34"xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://purl.uniprot.org/core/title | "GR SUMOylation and formation of an SUMO-SMRT/NCoR1-HDAC3 repressing complex is mandatory for GC-induced IR nGRE-mediated transrepression."xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://purl.uniprot.org/core/volume | "113"xsd:string |
| http://purl.uniprot.org/citations/26712002 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26712002 |
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| http://purl.uniprot.org/uniprot/#_E9Q9Y2-mappedCitation-26712002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26712002 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JED1-mappedCitation-26712002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26712002 |
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| http://purl.uniprot.org/uniprot/#_D3Z280-mappedCitation-26712002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26712002 |
| http://purl.uniprot.org/uniprot/#_D3Z2J5-mappedCitation-26712002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26712002 |
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| http://purl.uniprot.org/uniprot/#_E9Q701-mappedCitation-26712002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26712002 |
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| http://purl.uniprot.org/uniprot/#_F7C134-mappedCitation-26712002 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26712002 |