| http://purl.uniprot.org/citations/2674676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2674676 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2674676 | http://www.w3.org/2000/01/rdf-schema#comment | "The yeast gene RNA1 has been defined by the thermosensitive rna1-1 lesion. This lesion interferes with the processing and production of all major classes of RNA. Each class of RNA is affected at a distinct and presumably unrelated step. Furthermore, RNA does not appear to exit the nucleus. To investigate how the RNA1 gene product can pleiotropically affect disparate processes, we undertook a structural analysis of wild-type and mutant RNA1 genes. The wild-type gene was found to contain a 407-amino-acid open reading frame that encodes a hydrophilic protein. No clue regarding the function of the RNA1 protein was obtained by searching banks for similarity to other known gene products. Surprisingly, the rna1-1 lesion was found to code for two amino acid differences from wild type. We found that neither single-amino-acid change alone resulted in temperature sensitivity. The carboxy-terminal region of the RNA1 open reading frame contains a highly acidic domain extending from amino acids 334 to 400. We generated genomic deletions that removed C-terminal regions of this protein. Deletion of amino acids 397 to 407 did not appear to affect cell growth. Removal of amino acids 359 to 397, a region containing 24 acidic residues, caused temperature-sensitive growth. This allele, rna1-delta 359-397, defines a second conditional lesion of the RNA1 locus. We found that strains possessing the rna1-delta 359-397 allele did not show thermosensitive defects in pre-rRNA or pre-tRNA processing. Removal of amino acids 330 to 407 resulted in loss of viability."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.9.7.2989-2999.1989"xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.9.7.2989-2999.1989"xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/author | "Hopper A.K."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/author | "Hopper A.K."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/author | "Atkinson N.S."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/author | "Atkinson N.S."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/author | "Traglia H.M."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/author | "Traglia H.M."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/date | "1989"xsd:gYear |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/pages | "2989-2999"xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/pages | "2989-2999"xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/title | "Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/title | "Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes."xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/2674676 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2674676 |
| http://purl.uniprot.org/citations/2674676 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2674676 |
| http://purl.uniprot.org/citations/2674676 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2674676 |
| http://purl.uniprot.org/citations/2674676 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2674676 |