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http://purl.uniprot.org/citations/26792720http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/26792720http://www.w3.org/2000/01/rdf-schema#comment"

Background

Adiponectin (APN) is an adipocyte-derived bioactive molecule with anti-diabetic and anti-atherogenic properties. Although anti-diabetic effects are mostly mediated by the adiponectin receptors AdipoR1 and AdipoR2, the anti-atherogenic mechanisms have not been fully elucidated.

Methods and results

In this study, we identified E-selectin ligand (ESL)-1 as a novel APN-binding protein by mass spectrometry analysis of HepG2 cell-derived immunoprecipitant with an anti-APN antibody. Cell adhesion assays using fluorescence-labelled monocyte cell line THP-1 cells and human umbilical vein endothelial cells (HUVECs) revealed that APN-pre-treated THP-1 cells had reduced binding ability to HUVECs. This APN-mediated suppressive effect on monocyte binding to endothelial cells was partially abrogated by targeting ESL-1 with shRNA in THP-1 cells. In addition, serial mutagenesis analysis disclosed that five extracellular amino acids close to the N-terminus of ESL-1 were essential for binding with APN.

Conclusion

Our results highlight the fact that interaction between APN and ESL-1 could provide a fundamental mechanism underlying the anti-atherogenic properties of APN."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.org/dc/terms/identifier"doi:10.1016/j.bbrc.2016.01.023"xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Funahashi T."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Yamamoto H."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Matsumoto A."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Kihara S."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Shimomura I."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Kuroda N."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Uekita H."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Kochi I."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/author"Niinaga R."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/name"Biochem Biophys Res Commun"xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/pages"425-430"xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/title"E-selectin ligand-1 (ESL-1) is a novel adiponectin binding protein on cell adhesion."xsd:string
http://purl.uniprot.org/citations/26792720http://purl.uniprot.org/core/volume"470"xsd:string
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