| http://purl.uniprot.org/citations/26797706 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26797706 | http://www.w3.org/2000/01/rdf-schema#comment | "Adipogenic differentiation is characterized by an increase in two major transcription factors: peroxisome proliferator-activated receptor gamma (PPARγ) and the CCAAT/enhancer binding protein alpha (C/EBPα). These two signals are influenced by C/EBPβ and C/EBPδ and cross-regulate each other's expression during the initial stages of adipogenesis. Melatonin has been known to act as not only a direct scavenger of free radicals but also an inhibitor of glycogen synthase kinase 3β (GSK-3β). Here, we report that melatonin inhibits the adipogenic differentiation of human mesenchymal stem cells (hMSCs) which is due to the regulations of C/EBPβ in the early stage of adipogenic differentiation. Melatonin reduced the lipid accumulation, adiponectin, and lipoprotein lipase (LPL) during the adipogenic differentiation of hMSCs. Since C/EBPβ has been associated with the activation of PPARγ and the consensus site of ERK/GSK-3β, PPARγ and β-catenin were detected by immunofluorescence staining after pretreatment of melatonin. Melatonin blocked the activation of PPARγ which induced the degradation of β-catenin. Melatonin also decreased the levels of cyclic adenosine-3,5-monophosphate (cAMP) and reactive oxygen species (ROS). The cAMP triggered the activity of C/EBPβ which is a critical inducer of PPARγ and C/EBPα activation in the early stage of adipogenic differentiation, and this is further affected by ROS production. The adipogenic marker proteins such as PPARγ, C/EBPα, C/EBPβ, and pERK were also decreased by melatonin. In summary, melatonin inhibited the cAMP synthesis through ROS reduction and the phosphorylation of the ERK/GSK-3β site which is known to be responsible for C/EBPβ activation for adipogenic differentiation in hMSCs."xsd:string |
| http://purl.uniprot.org/citations/26797706 | http://purl.org/dc/terms/identifier | "doi:10.1007/s13105-015-0463-3"xsd:string |
| http://purl.uniprot.org/citations/26797706 | http://purl.uniprot.org/core/author | "Rhee Y.H."xsd:string |
| http://purl.uniprot.org/citations/26797706 | http://purl.uniprot.org/core/author | "Ahn J.C."xsd:string |
| http://purl.uniprot.org/citations/26797706 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26797706 | http://purl.uniprot.org/core/name | "J Physiol Biochem"xsd:string |
| http://purl.uniprot.org/citations/26797706 | http://purl.uniprot.org/core/pages | "145-155"xsd:string |
| http://purl.uniprot.org/citations/26797706 | http://purl.uniprot.org/core/title | "Melatonin attenuated adipogenesis through reduction of the CCAAT/enhancer binding protein beta by regulating the glycogen synthase 3 beta in human mesenchymal stem cells."xsd:string |
| http://purl.uniprot.org/citations/26797706 | http://purl.uniprot.org/core/volume | "72"xsd:string |
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