| http://purl.uniprot.org/citations/26814197 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26814197 | http://www.w3.org/2000/01/rdf-schema#comment | "Members of the poly-ADP-ribose polymerase (PARP) family catalyse the ADP-ribosylation of target proteins and are known to play important roles in many cellular processes, including DNA repair, differentiation and transcription. The majority of PARPs exhibit mono-ADP-ribosyltransferase activity rather than PARP activity; however, little is known about their biological activity. In the present study, we report that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly-ADP-ribose polymerase (TIPARP), mono-ADP-ribosylates and positively regulates liver X receptor α (LXRα) and LXRβ activity. Overexpression of TIPARP enhanced LXR-reporter gene activity. TIPARP knockdown or deletion reduced LXR regulated target gene expression levels in HepG2 cells and in Tiparp(-/-)mouse embryonic fibroblasts (MEFs) respectively. Deletion and mutagenesis studies showed that TIPARP's zinc-finger and catalytic domains were required to enhance LXR activity. Protein interaction studies using TIPARP and LXRα/β peptide arrays revealed that LXRs interacted with an N-terminal sequence (a.a. 209-236) of TIPARP, which also overlapped with a putative co-activator domain of TIPARP (a.a. 200-225). Immunofluorescence studies showed that TIPARP and LXRα or LXRβ co-localized in the nucleus.In vitroribosylation assays provided evidence that TIPARP mono-ADP-ribosylated both LXRα and LXRβ. Co-immunoprecipitation (co-IP) studies revealed that ADP-ribosylase macrodomain 1 (MACROD1), but not MACROD2, interacted with LXRs in a TIPARP-dependent manner. This was complemented by reporter gene studies showing that MACROD1, but not MACROD2, prevented the TIPARP-dependent increase in LXR activity. GW3965-dependent increases in hepatic Srebp1 mRNA and protein expression levels were reduced in Tiparp(-/-)mice compared with Tiparp(+/+)mice. Taken together, these data identify a new mechanism of LXR regulation that involves TIPARP, ADP-ribosylation and MACROD1."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20151077"xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "MacPherson L."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "Tan S."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "Cho T."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "Matthews J."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "Nebb H.I."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "Tamblyn L."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "Bindesboll C."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "Bott D."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/author | "Gronning-Wang L."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/pages | "899-910"xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/title | "TCDD-inducible poly-ADP-ribose polymerase (TIPARP/PARP7) mono-ADP-ribosylates and co-activates liver X receptors."xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://purl.uniprot.org/core/volume | "473"xsd:string |
| http://purl.uniprot.org/citations/26814197 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26814197 |
| http://purl.uniprot.org/citations/26814197 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26814197 |
| http://purl.uniprot.org/uniprot/#_D3Z7U2-mappedCitation-26814197 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26814197 |
| http://purl.uniprot.org/uniprot/#_A0A140LHK8-mappedCitation-26814197 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26814197 |
| http://purl.uniprot.org/uniprot/#_A0A140LHL9-mappedCitation-26814197 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26814197 |
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| http://purl.uniprot.org/uniprot/#_A0A140LHY6-mappedCitation-26814197 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26814197 |
| http://purl.uniprot.org/uniprot/#_A0A140LIH8-mappedCitation-26814197 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26814197 |