| http://purl.uniprot.org/citations/26819475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26819475 | http://www.w3.org/2000/01/rdf-schema#comment | "The anti-angiogenic protein, soluble fms-like tyrosine kinase-1 (sFLT-1), plays a central role in preeclamptic pathophysiology. A splice variant of FLT-1 (VEGF receptor 1), sFLT-1 is released in excessive amounts from the preeclamptic placenta into the maternal circulation, where it causes endothelial dysfunction manifesting as end-organ disease. However, the mechanisms regulating its production within the placenta remain poorly understood. Recently it was shown in endothelial cells that Jumonji domain containing protein 6 (JMJD6) hydroxylates U2 small nuclear ribonucleoprotein auxiliary factor 65-kDa subunit (U2AF65, a component of the splicesome). The hydroxylation by JMJD6 is oxygen dependent. Under hypoxia, JMJD6 is less able to hydroxylate U2AF65, and this unhydroxylated form of U2AF65 biases splicing of FLT-1 to sFLT-1. We assessed whether oxygen-sensing JMJD6 is differentially expressed in preeclamptic placenta and regulates sFLT-1 splicing in placenta via U2AF65. JMJD6 protein expression was significantly reduced in preterm preeclamptic placenta (P < 0.0001; n = 21) relative to preterm controls (n = 10). Exposing both placental and endothelial cells to hypoxia significantly reduced JMJD6 mRNA and increased sFLT-1 mRNA and protein expression. Silencing JMJD6 in primary endothelial and trophoblast cells significantly increased sFLT-1 secretion. Next, we examined whether these molecules may be directly interacting. We demonstrated that placental U2AF65 colocalized with JMJD6. In turn, we found JMJD6 directly interacts with U2AF65, which in turn produces sFLT-1 mRNA transcripts. Taken together, our findings provide evidence that JMJD6 may play a role in regulating the production of sFLT-1 in the preeclamptic placenta. Decreased placental JMJD6 expression may be an important component to the pathophysiology of preeclampsia."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.org/dc/terms/identifier | "doi:10.1095/biolreprod.115.134460"xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Tong S."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Ye L."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Palmer K.R."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Cannon P."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Hannan N.J."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Kaitu'u-Lino T.J."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Tuohey L."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Illanes S.E."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/author | "Brownfoot F.C."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/name | "Biol Reprod"xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/pages | "59"xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/title | "Jumonji Domain Containing Protein 6 Is Decreased in Human Preeclamptic Placentas and Regulates sFLT-1 Splice Variant Production."xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://purl.uniprot.org/core/volume | "94"xsd:string |
| http://purl.uniprot.org/citations/26819475 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26819475 |
| http://purl.uniprot.org/citations/26819475 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26819475 |
| http://purl.uniprot.org/uniprot/#_A0A0S2PZM4-mappedCitation-26819475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26819475 |
| http://purl.uniprot.org/uniprot/#_B2WTI3-mappedCitation-26819475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26819475 |
| http://purl.uniprot.org/uniprot/#_B2WTI4-mappedCitation-26819475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26819475 |
| http://purl.uniprot.org/uniprot/#_H9N1E7-mappedCitation-26819475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26819475 |
| http://purl.uniprot.org/uniprot/#_H9N1E8-mappedCitation-26819475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26819475 |
| http://purl.uniprot.org/uniprot/#_B5A924-mappedCitation-26819475 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/26819475 |