| http://purl.uniprot.org/citations/26826386 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26826386 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/26826386 | http://www.w3.org/2000/01/rdf-schema#comment | "Escherichia coli is naturally transformable, independent on the conserved DNA uptake machinery for single-stranded DNA (ssDNA) integration. The transfer of double-stranded DNA (dsDNA) during natural transformation of E. coli is regulated by the alternative sigma factor σ(S). However, it remains mysterious how dsDNA transfers across the membranes and how σ(S) regulates natural transformation of E. coli. Here, I screened for σ(S)-regulated genes for dsDNA transfer in E. coli. The screening identified the σ(S)-regulated genes ydcS and ydcV, both locate on the putative ABC transporter ydcSTUV operon. Considering that ydcS and ydcV are predicted to encode a periplasmic protein and an inner membrane protein for substrate binding and translocation respectively, I propose that they may mediate dsDNA translocation across the inner membrane during natural transformation. In chemical transformation of E. coli, ydcS was but ydcV was not required. Thus, YdcV should not be the channel for dsDNA translocation in artificial transformation. Together with the previous observation that the outer membrane porin OmpA mediates dsDNA transfer across the outer membrane in chemical transformation but not in natural transformation, I conclude that dsDNA transfers across the two membranes through different routes in natural and artificial transformation of E. coli."xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2016.01.137"xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2016.01.137"xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/author | "Sun D."xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/author | "Sun D."xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/pages | "213-218"xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/pages | "213-218"xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/title | "Two different routes for double-stranded DNA transfer in natural and artificial transformation of Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/title | "Two different routes for double-stranded DNA transfer in natural and artificial transformation of Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/volume | "471"xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://purl.uniprot.org/core/volume | "471"xsd:string |
| http://purl.uniprot.org/citations/26826386 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26826386 |
| http://purl.uniprot.org/citations/26826386 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/26826386 |
| http://purl.uniprot.org/citations/26826386 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26826386 |
| http://purl.uniprot.org/citations/26826386 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/26826386 |
| http://purl.uniprot.org/uniprot/P77795 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/26826386 |
| http://purl.uniprot.org/uniprot/P0AFR9 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/26826386 |
| http://purl.uniprot.org/uniprot/P76108 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/26826386 |
| http://purl.uniprot.org/uniprot/P77156 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/26826386 |